Localization of androgen-binding protein in proliferating Sertoli cells in culture.
Localization of androgen-binding protein in proliferating Sertoli cells in culture.
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DOI：
10.1073/pnas.77.9.5322
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发表时间：
1980-09
影响因子：
11.1
通讯作者：
A. Kierszenbaum;M. Feldman;O. Lea;W. A. Spruill;L. Tres;P. Petrusz;F. S. French
中科院分区：
文献类型：
--
作者：
A. Kierszenbaum;M. Feldman;O. Lea;W. A. Spruill;L. Tres;P. Petrusz;F. S. French
在分离自20- 22日龄大鼠的培养支持细胞中，研究了雄激素结合蛋白（ABP）的过氧化物酶和免疫荧光定位模式，ABP是支持细胞功能的生物标志物。在支持细胞中观察到ABP免疫反应性，其形式为直径可变的细胞质颗粒，具有特征性脂滴和集落形成的上皮样生长模式。用[3 H]胸苷孵育培养物证明，支持细胞继续产生ABP，同时保留其合成DNA和进行有丝分裂的能力。暴露于促卵泡素（卵泡刺激素）和作用于环核苷酸代谢的药物后，培养的支持细胞数量不等，发生形态学转化。支持细胞形状的诱导变化与含ABP颗粒从细胞质中消失相一致。这些观察结果表明，ABP的免疫学定位技术是一个有价值的工具，支持细胞的结构和功能特性的表征在培养中。
The peroxidase and immunofluorescent localization patterns of androgen-binding protein (ABP), a biological marker of Sertoli cell function, have been examined in cultured Sertoli cells isolated from 20- to 22-day-old rats. ABP immunoreactivity in the form of cytoplasmic granules of variable diameter was observed in Sertoli cells with characteristic lipid droplets and a colony-forming, epithelial-like growth pattern. Incubation of cultures with [3H]thymidine demonstrated that Sertoli cells continue to produce ABP while retaining their capability for synthesizing DNA and undergoing mitosis. A variable number of cultured Sertoli cells became morphologically transformed after exposure to follitropin (follicle-stimulating hormone) and pharmacological agents acting on cyclic nucleotide metabolism. The induced change in Sertoli cell shape coincided with a disappearance of ABP-containing granules from the cytoplasm. These observations demonstrate that localization of ABP by immunological techniques is a valuable tool for the characterization of structural and functional properties of Sertoli cell in culture.