Invasion of human coronary artery cells by periodontal pathogens
Invasion of human coronary artery cells by periodontal pathogens
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DOI：
10.1128/iai.67.11.5792-5798.1999
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发表时间：
1999-11-01
影响因子：
3.1
通讯作者：
Progulske-Fox, A
中科院分区：
文献类型：
--
作者：
Dorn, BR;Dunn, WA;Progulske-Fox, A
有一个新兴的范式转变，从冠心病有一个纯粹的遗传和营养的原因，可能有一个感染性病因。最近的流行病学研究表明，牙周病和冠心病之间的相关性。然而，迄今为止，关于这种关联的可能疾病机制的信息很少。我们的假设是，口腔细菌对冠状动脉细胞的侵入可能启动和/或加剧动脉粥样硬化的炎症反应。由于一些牙周病原体已被报道侵入口腔上皮组织，我们测试的能力，三个假定的牙周pahogens-Eikenella corrodens，牙龈卟啉单胞菌，和Prevotella intermedia-to侵入人冠状动脉内皮细胞和冠状动脉平滑肌细胞。在这项研究中，我们证明了通过抗生素保护试验和电子显微镜，特定的物种和菌株侵入冠状动脉细胞在一个显着的水平。肌动蛋白聚合和真核生物蛋白质合成代谢活跃的细胞所需的，因为相应的抑制剂几乎废除入侵。通过形态学分析，发现许多细胞内牙龈卟啉单胞菌生物体存在于类似自噬体的多膜空泡中。这是第一次报告口腔微生物侵入人血管原代细胞培养。
There is an emerging paradigm shift from coronary heart disease having a purely hereditary and nutritional causation to possibly having an infectious etiology. Recent epidemiological studies have shown a correlation between periodontal disease and coronary heart disease. However, to date, there is minimal information as to the possible disease mechanisms of this association. It is our hypothesis that invasion of the coronary artery cells by oral bacteria may start and/or exacerbate the inflammatory response in atherosclerosis. Since a few periodontal pathogens have been reported to invade oral epithelial tissues, we tested the ability of three putative periodontal pahogens-Eikenella corrodens, Porphyromonas gingivalis, and Prevotella intermedia-to invade human coronary artery endothelial cells and coronary artery smooth muscle cells. In this study we demonstrate by an antibiotic protection assay and electron microscopy that specific species and strains invade coronary artery cells at a significant level. Actin polymerization and eukaryotic protein synthesis in metabolically active cells were required since the corresponding inhibitors nearly abrogated invasion. Many intracellular P. gingivalis organisms were seen to be present in multimembranous vacuoles resembling autophagosomes by morphological analysis. This is the first report of oral microorganisms invading human primary cell cultures of the vasculature.