Mesenchymal and neural stem cells labeled with HEDP-coated SPIO nanoparticles: In vitro characterization and migration potential in rat brain
Mesenchymal and neural stem cells labeled with HEDP-coated SPIO nanoparticles: In vitro characterization and migration potential in rat brain
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DOI：
10.1016/j.brainres.2008.12.013
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发表时间：
2009-02-19
期刊：
影响因子：
2.9
通讯作者：
Montero-Menei, Claudia N.
中科院分区：
文献类型：
--
作者：
Delcroix, Gaetan. J-R.;Jacquart, Matthieu;Montero-Menei, Claudia N.
间充质干细胞（MSC）可以在神经源性微环境中存在的基质分子和生长因子的影响下在体外转分化为神经细胞。然而，有关此类干细胞行为的进一步体内实验仍有待完成。在这项研究中，大鼠 MSC (rMSC) 被移植到大鼠大脑的神经源性环境中，即室下区 (SVZ)，以便使用超顺磁性氧化铁 (SPIO) 纳米粒子检测和跟踪它们的迁移。我们试图表征铁负荷对 rMSC 行为的潜在影响，并解决 rMSC 在暴露于足够的脑微环境时迁移的潜力。 1-羟基亚乙基-1.1-二膦酸 (HEDP) 包被的 SPIO 纳米颗粒可有效标记 rMSC，且不会对细胞活力和体外分化潜力产生显着不利影响。与用作阳性对照的铁标记的大鼠神经干细胞（rNSC）相反，铁标记的rMSC对体内SVZ微环境没有反应并且不会迁移，除非对嗅球进行机械损伤。这证实了铁标记的 rMSC 向病变迁移的已知潜力，据我们所知，这是第一项描述从 SVZ 通过吻侧迁移流 (RMS) 向嗅球进行如此长距离迁移的研究。 (C) 2008 Elsevier B.V. 保留所有权利。
Mesenchymal stem cells degrees MSC) may trans differentiate into neural cells in vitro under the influence of matrix molecules and growth factors present in neurogenic niches. However, further experiments on the behavior of such stem cells remain to be done in vivo. In this study, rat MSC (rMSC) have been grafted in a neurogenic environment of the rat brain, the subventricular zone (SVZ), in order to detect and follow their migration using superparamagnetic iron oxide (SPIO) nanoparticles. We sought to characterize the potential effect of iron loading on the behavior of rMSC as well as to address the potential of rMSC to migrate when exposed to the adequate brain microenvironment. 1-hydroxyethylidene-1.1-bisphosphonic acid (HEDP)-coated SPIO nanoparticles efficiently labeled rMSC without significant adverse effects on cell viability and on the in vitro differentiation potential. in opposition to iron-labeled rat neural stem cells (rNSC), used as a positive control, iron-labeled rMSC did not respond to the SVZ microenvironment in vivo and did not migrate, unless a mechanical lesion of the olfactory bulb was performed. This confirmed the known potential of iron-labeled rMSC to migrate toward lesions and, as far as we know, this is the first study describing such a long distance migration from the SVZ toward the olfactory bulb through the rostral migratory stream (RMS). (C) 2008 Elsevier B.V. All rights reserved.