Noninvasive assessment of human embryo nutrient consumption as a measure of developmental potential
Noninvasive assessment of human embryo nutrient consumption as a measure of developmental potential
复制标题
DOI：
10.1016/s0015-0282(01)02888-6
复制
发表时间：
2001-12-01
影响因子：
6.7
通讯作者：
Schoolcraft, WB
中科院分区：
文献类型：
--
作者：
Gardner, DK;Lane, M;Schoolcraft, WB
目的：探讨胚泡发育与形态和胚胎代谢的关系。设计：对单个胚胎的碳水化合物摄取和氨的产生进行非侵入性评估。单位：私人辅助生殖技术单位。患者(S)：患者捐献，征得患者同意，干预(S)：60枚解冻的原核胚胎在连续培养至囊胚期，同时对胚胎代谢进行无创性分析，并对13枚未冷冻胚胎的囊胚进行分析。主要观察指标(S)：丙酮酸和葡萄糖的消耗以及囊胚的形成和质量。结果(S)：继续形成囊胚的胚胎在第4天的丙酮酸和葡萄糖的摄取量显著高于未发育到囊胚期的胚胎。最高级别的胚泡对葡萄糖的摄取最大，而丙酮酸的摄取与胚泡级别相似，这表明葡萄糖是人类胚泡更重要的营养物质。在来自同一患者的相同级别的囊胚中，有相当大的葡萄糖消耗扩散，这表明葡萄糖消耗可能用于识别要转移的囊胚。此外，还测量了单个胚胎的铵生成量，反映了人类胚胎对氨基酸的转氨和利用。结论：在培养中识别最具发育潜力的胚胎的能力将有助于向单胚胎移植的转移。(Fertil Steril(R)2001；76：1175-80。(C)2001年美国生殖医学会。)。
Objective: To determine the relationship between blastocyst development and morphology and embryo metabolism.Design: Noninvasive assessment of carbohydrate uptake and ammonium production by individual embryos.Setting: Private assisted reproductive technology unit.Patient(s): Patients donated, with consent, cryopreserved pronucleate embryos and noncryopreserved blastocysts.Intervention(s): Culture of 60 thawed pronucleate embryos in sequential media to the blastocyst stage with concomitant noninvasive analysis of embryo metabolism and analysis of 13 blastocysts from noncryopreserved embryos.Main Outcome Measure(s): Pyruvate and glucose consumption as well as blastocyst formation and quality.Result(s): Pyruvate and glucose uptakes on day 4 were significantly higher by embryos that went on to form blastocysts than by embryos that failed to develop to the blastocyst stage. Glucose uptakes were greatest in those blastocysts of highest grade, whereas pyruvate uptakes were similar irrespective of blastocyst grade, indicating that glucose is the more important nutrient for the human blastocyst. Among blastocysts of the same grade from the same patient, there was considerable spread of glucose consumption, indicating that glucose consumption may be of use in identifying blastocysts for transfer. Ammonium production by individual embryos was also measured, reflecting amino acid transamination and use by the human embryo.Conclusion(s): The ability to identify in culture the embryo with the highest developmental potential will facilitate the move to single-embryo transfers. (Fertil Steril (R) 2001;76:1175-80. (C) 2001 by American Society for Reproductive Medicine.).