Secretion of Porphyromonas gingivalis fimbrillin polypeptides by recombinant Streptococcus gordonii.
Secretion of Porphyromonas gingivalis fimbrillin polypeptides by recombinant Streptococcus gordonii.
复制标题
重组戈登链球菌分泌牙龈卟啉单胞菌纤毛蛋白多肽。
DOI：
10.1006/bbrc.1997.7306
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发表时间：
1997
影响因子：
3.1
通讯作者：
Genco,RJ
中科院分区：
文献类型：
--
作者：
Sharma,A;Sojar,HT;Hruby,DE;Kuramitsu,HK;Genco,RJ
牙龈卟啉单胞菌的菌毛在牙周病的发病中起着重要作用。一个结构亚基。牙龈菌毛（fimbrillin）已显示促进细菌粘附于宿主表面，并且还诱导免疫应答。已经确定了负责粘附或引发免疫应答的菌毛蛋白的生物活性结构域。在以前的研究中，我们设计了人类口腔微生物戈登链球菌，使其在细菌表面表达这种生物活性结构域，作为疫苗递送系统。在这项研究中，我们报告了一种替代的方法分泌菌毛蛋白多肽结构域到介质中的表面表达系统的修改之前所描述的。这样的重组体。gordonii除了作为抗原呈递的来源以触发保护性免疫应答之外，还可能具有直接阻断菌毛介导的P粘附的额外优势。牙龈炎在植入后进入口腔。这种方法也可用于在粘膜表面上分泌其他生物学上重要的治疗分子，以调节局部微环境。
The fimbriae ofPorphyromonas gingivalisplays an important role in the pathogenesis of periodontal disease. A structural subunit of theP. gingivalisfimbriae, fimbrillin, has been shown to promote adherence of the bacteria to host surfaces and also induce an immune response. Biologically active domains of fimbrillin responsible for adherence or eliciting immune responses have been determined. In a previous study, we engineered the human oral commensal organismStreptococcus gordoniito express such biologically active domains on the surface of the bacteria as a vaccine delivery system. In this study we report an alternative approach of secreting fimbrillin polypeptide domains into the medium by modification of the surface-expression system described earlier. Such recombinantS. gordonii,in addition to being a source for antigen presentation to trigger a protective immune response, may have the added advantage of directly blocking the fimbriae-mediated adherence ofP. gingivalisto the oral cavity following implantation. This approach can also be utilized for secreting other biologically important therapeutic molecules on mucosal surfaces for modulating local microenvironments.