Comparative study of intracavernous pressure and cavernous pathology after bilateral cavernous nerve crushing and resection in rats.
Comparative study of intracavernous pressure and cavernous pathology after bilateral cavernous nerve crushing and resection in rats.
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大鼠双侧海绵体神经粉碎切除术后海绵体压力与海绵体病理的对比研究
DOI：
10.4103/aja.aja_10_20
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发表时间：
2020-11
影响因子：
2.9
通讯作者：
Guan RL
中科院分区：
文献类型：
--
作者：
Li M;Yuan YM;Yang BC;Gu SJ;Li HX;Xin ZC;Fang D;Guan RL
本研究旨在比较双侧海绵体神经挤压(BCNC)和双侧海绵体神经切除(BCNR)对大鼠海绵体内压(ICP)和海绵体病理的影响，探讨双侧海绵体神经挤压和BCNR模型的最佳治疗时机。12周龄雄性大鼠72只，随机分为3组：假手术组(只暴露双侧海绵体神经)、BCnc组(挤压BCN 2min)和BCNR组(切除BCN5 mm)。分别于神经损伤后1周、3周、5周测定阴茎勃起功能，取阴茎组织进行免疫组织化学、免疫荧光、Western印迹和细胞因子阵列分析。结果发现，BCNR和BNC术后1周和3周的勃起功能参数，包括最大值、面积、斜率和平均动脉压(MAP)均显著降低。术后5周，BCNR组与Sham组之间的ICP/MAP差异无统计学意义，而BCNR组的ICP/MAP仍显著低于Sham组。BCNC和BCNR后，神经元型一氧化氮合酶阳性纤维、血管内皮细胞和平滑肌细胞数量减少，而III型胶原含量增加。随着时间的推移，这些病理改变逐渐恢复，尤其是在BCnc组。我们的研究结果表明，BCNC会导致急性和可逆性勃起功能障碍，因此治疗时间应限制在BCNC后的前3周。相比之下，BCNR模型的自愈能力较差，更适合长期治疗研究。
This study aimed to compare the effects of bilateral cavernous nerve crushing (BCNC) and bilateral cavernous nerve resection (BCNR) on intracavernous pressure (ICP) and cavernous pathology in rats and to explore the optimal treatment time for the BCNC and BCNR models. Seventy-two male rats aged 12 weeks were randomly divided into three equal groups: Sham (both cavernous nerves exposed only), BCNC (BCN crushed for 2 min), and BCNR (5 mm of BCN resected). Erectile function was then measured at 1 week, 3 weeks, and 5 weeks after nerve injury, and penile tissues were harvested for histological and molecular analyses by immunohistochemistry, immunofluorescence, Western blot, and cytokine array. We found that erectile function parameters including the maximum, area, and slope of ICP/mean arterial pressure (MAP) significantly decreased after BCNR and BCNC at 1 week and 3 weeks. At 5 weeks, no significant differences were observed in ICP/MAP between the BCNC and Sham groups, whereas the ICP/MAP of the BCNR group remained significantly lower than that of the Sham group. After BCNC and BCNR, the amount of neuronal-nitric oxide synthase-positive fibers, smooth muscle cells, and endothelial cells decreased, whereas the amount of collagen III content increased. These pathological changes recovered over time, especially in the BCNC group. Our findings demonstrate that BCNC leads to acute and reversible erectile dysfunction, thus treatment time should be restricted to the first 3 weeks post-BCNC. In contrast, the self-healing ability of the BCNR model is poor, making it more suitable for long-term treatment research.
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影响因子：
2.1
作者：
Ferrini, Monica G.;Davila, Hugo H.;Rajfer, Jacob
通讯作者：
Rajfer, Jacob
影响因子：
6.6
作者：
Kendirci, M;Zsengellér, Z;Hellstrom, WJG
通讯作者：
Hellstrom, WJG
影响因子：
6.6
作者：
Canguven, Onder;Lagoda, Gwen;Burnett, Arthur L.
通讯作者：
Burnett, Arthur L.
源自间充质干细胞的外泌体在大鼠海绵体神经损伤模型中发挥治疗作用。
影响因子：
4.5
作者：
Li, M.;Lei, H.;Guan, R.
通讯作者：
Guan, R.
影响因子：
3.2
作者：
Weyne, Emmanuel;Mulhall, John;Albersen, Maarten
通讯作者：
Albersen, Maarten