Keratin 7 expression in colorectal cancer - freak of nature or significant finding?
Keratin 7 expression in colorectal cancer - freak of nature or significant finding?
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DOI：
10.1111/j.1365-2559.2011.03694.x
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发表时间：
2011-08-01
期刊：
影响因子：
6.4
通讯作者：
Langner, Cord
中科院分区：
文献类型：
--
作者：
Harbaum, Lars;Pollheimer, Marion J.;Langner, Cord
目的：评估结直肠癌中角蛋白 7 (K7) 表达的普遍性，并将结果与临床病理参数和患者结果相关联。方法和结果：使用组织微阵列技术通过免疫组织化学评估了总共 370 名患者的 K7 表达。 K7 表达按任一焦点进行半定量评分（50%）。总共有 32 个 (9%) 肿瘤对 K7 呈免疫反应，其中 5 个病例分别显示广泛表达、4 个中度表达和 23 个局灶表达。 K7 表达与肿瘤分化差（P = 0.005）和肿瘤出芽程度（P = 0.02）相关。在整个切片中，K7 免疫反应性在侵袭前沿的单个细胞和小细胞群中占主导地位。连续切片分析表明，K7 阳性细胞与角蛋白 20 共定位，但缺乏 E-钙粘蛋白、MUC2 和 MIB-1 的免疫反应性。 K7 阳性肿瘤患者中有 52% 发生疾病进展，K7 阴性肿瘤患者中有 41% 发生疾病进展（P = 0.19）； 48% 的 K7 阳性肿瘤患者死于疾病，但只有 33% 的 K7 阴性肿瘤患者死于疾病 (P = 0.06)。 结论：出芽癌细胞中 K7 的异常表达代表上皮表型的改变（“上皮-上皮转变”：EET），这可能与运动性和侵袭潜力的增加有关。
Aims: To assess the prevalence of keratin 7 (K7) expression in colorectal cancer and to correlate findings with clinicopathological parameters and patients' outcome.Method and results: A total of 370 patients were evaluated for K7 expression by immunohistochemistry using a tissue microarray technique. K7 expression was scored semiquantitatively as either focal (50%). In all, 32 (9%) tumours were immunoreactive for K7, with five cases showing extensive, four moderate and 23 focal expression, respectively. K7 expression was associated with poor tumour differentiation (P = 0.005) and the extent of tumour budding (P = 0.02). In whole sections, K7 immunoreactivity prevailed in single cells and small clusters of cells at the invasion front. Analysis of serial sections showed that K7-positive cells colocalized with keratin 20, whereas they lacked immunoreactivity for E-cadherin, MUC2 and MIB-1. Disease progression occurred in 52% of patients with K7-positive tumours and 41% with K7-negative tumours (P = 0.19); 48% of patients with K7-positive tumours but only 33% with K7-negative tumours died of disease (P = 0.06).Conclusions: Aberrant expression of K7 in budding cancer cells represents a modification of the epithelial phenotype ('epithelial-epithelial transition': EET) which may be linked to gains in motility and invasive potential.