The effect of tracheal occlusion on lung branching in the rat nitrofen CDH model
The effect of tracheal occlusion on lung branching in the rat nitrofen CDH model
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DOI：
10.1016/j.jss.2007.07.019
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发表时间：
2008-08-01
影响因子：
2.2
通讯作者：
Laberge, Jean-Martin
中科院分区：
文献类型：
--
作者：
Baird, Robert;Ehan, Nasir;Laberge, Jean-Martin
背景。胎儿气管阻塞（TO）已被研究作为继发于先天性膈疝（CDH）的肺发育不全的治疗选择。 TO 增加了肺的大小，但尚不清楚 TO 是否会刺激成熟的肺生长，或者只是诱导肺泡化而不伴随支气管发育。在这项研究中，我们通过传统的组织学和形态测量分析以及肺铸型来描述患有 CDH 的胎鼠（伴或不伴 TO）的支气管分支发育特征。材料和方法。大鼠母鼠在妊娠第 9.5 天被灌胃除草醚，每只母鼠的 3 至 4 个胎儿在妊娠第 19 天（足月 = 22 天）接受胎儿 TO。第21天处死胎儿，确认CDH的存在，并计算肺重量与体重之比(LW/BW)。所有研究组的肺模型均使用液体硅制作，并对从肺外周到隆突的支气管分支进行量化。结果。与对照组相比，CDH 胎儿的 LW/BW 比率较小，空域百分比 (%) 较小，并且肺分支较少。 TO 处理的胎儿具有更大的 LW/BW 比率和 % 空域，但没有不同数量的分支迭代。患有 CDH 和 TO 的胎儿的 LW/BW 比率恢复到对照水平 (P = 0.42)，但支气管分支迭代次数仍然少于对照动物。结论。这项研究的结果表明，在妊娠第 19 天的动物模型中，TO 促进远端气道增殖，但不能逆转 CDH 导致的肺发育不全中出现的支气管分支发育不良。 (C) 2008 Elsevier Inc. 保留所有权利。
Background. Fetal tracheal occlusion (TO) has been investigated as a treatment option for lung hypoplasia secondary to congenital diaphragmatic hernia (CDH). TO increases lung size, but it is unclear whether TO stimulates mature lung growth or simply induces alveolarization without concomitant bronchial development. In this study, we characterize bronchial branch development in fetal rats with CDH with or without TO through conventional histological and morphometric analysis as well as lung casting.Materials and methods. Rat dams were gavaged nitrofen at gestational day 9.5, and 3 to 4 fetuses per dam underwent fetal TO on gestational day 19 (term = 22 days). Fetuses were sacrificed on day 21, the presence of CDH was confirmed, and the lung weight to body weight ratio (LW/BW) was calculated. Lung casts of all research groups were created using liquid silicon and bronchial branches were quantified from lung periphery to carina.Results. CDH fetuses had smaller LW/BW ratios and a lesser percentage (%) of airspace when compared to controls, and manifested less lung branching than controls. Fetuses treated by TO had a greater LW/BW ratio and % airspace, but did not have a different number of branch iterations. Fetuses with CDH and TO demonstrated a restoration in LW/BW ratio to control levels (P = 0.42), but the number of bronchial branch iterations remained less than control animals.Conclusion. The results of this study suggest that TO in this animal model at gestational day 19 promotes distal airway proliferation but does not reverse the under-development of bronchial branching seen in lung hypoplasia due to CDH. (C) 2008 Elsevier Inc. All rights reserved.