Adaptive optics with combined optical coherence tomography and scanning laser ophthalmoscopy for in vivo mouse retina imaging
Adaptive optics with combined optical coherence tomography and scanning laser ophthalmoscopy for in vivo mouse retina imaging
复制标题
自适应光学结合光学相干断层扫描和扫描激光检眼镜用于小鼠体内视网膜成像
DOI：
10.1117/12.2288607
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Manns, Fabrice
中科院分区：
文献类型：
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作者：
Zhang, Pengfei;Wahl, Daniel J.;Mocci, Jacopo;Manna, Suman K.;Meleppat, Ratheesh K.;Bonora, Stefano;Sarunic, Marinko V.;Pugh, Edward N.;Zawadzki, Robert J.;Manns, Fabrice
光学相干断层扫描（OCT）和扫描激光检眼镜（SLO）是两种最先进的成像技术，通常用于研究视网膜。自适应光学（AO）方法能够实现眼睛像差的高保真度校正，从而提高SLO和OCT系统的分辨率和灵敏度。在这里，我们目前的工作集成到先前描述的小鼠视网膜AO-SLO系统的OCT，允许同时反射和荧光成像。新系统允许同时采集AO-SLO和AO-OCT数据，便于两种模式的数据配准和比较。该系统具有200 kHz A扫描/像素的数据采集速度和高体积分辨率。
Optical coherence tomography (OCT) and scanning laser ophthalmoscopy (SLO) are two state-of-the-art imaging technologies commonly used to study retina. Adaptive Optics (AO) methodologies enable high-fidelity correction of ocular aberrations, resulting in improved resolution and sensitivity for both SLO and OCT systems. Here we present work integrating OCT into a previously described mouse retinal AO-SLO system, allowing simultaneous reflectance and fluorescence imaging. The new system allows simultaneous data acquisition of AO-SLO and AO-OCT, facilitating registration and comparison of data from both modalities. The system has data acquisition speed of 200 kHz A-scans/pixel, and high volumetric resolution.