BrKCS6 mutation conferred a bright glossy phenotype to Chinese cabbage
BrKCS6 mutation conferred a bright glossy phenotype to Chinese cabbage
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DOI：
10.1007/s00122-023-04464-1
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发表时间：
2023-10-01
影响因子：
5.4
通讯作者：
Feng，Hui
中科院分区：
文献类型：
--
作者：
Song，Gengxing;Dong，Shiyao;Feng，Hui
通过MutMap和KASP分析，克隆了编码3-酮脂酰辅酶A脱氢酶的关键信息基因BrKCS 6，并通过等位突变体验证了其在大白菜中的功能。摘要叶菜类蔬菜的绿色外观是一种有吸引力的商品性状，主要是由于表皮蜡晶的缺失。本研究从大白菜EMS诱变群体中获得了两个外表皮蜡晶缺失等位突变体wdm 9和wdm 10。北京亚种）。遗传分析表明，该突变体的表型受隐性核基因控制，MutMap和KASP分析表明，编码3-酮脂酰辅酶A酶的BrKCS 6为候选基因。wdm 9中BrKCS 6的一个SNP（G → A）导致丝氨酸（S）被苯丙氨酸（F）取代，wdm 10中BrKCS 6的另一个SNP（G → A）导致丝氨酸（S）被亮氨酸（L）取代。两个SNP均位于ACP_syn_III_C保守结构域，对应于BrKCS 6及其同源物中的两个高度保守位点。这两个氨基酸取代改变了BrKCS 6蛋白的二级结构和三维结构。qRT-PCR结果表明，突变体中BrKCS 6在花、茎、叶中的相对表达量显著降低，下游关键基因的相对表达量也下调。本研究首次克隆了大白菜珍贵的光泽基因BrKCS 6，该基因在大白菜商品品质育种中具有很大的应用潜力。
Key messageBrKCS6encoding 3-ketoacyl-CoA synthases was cloned through MutMap and KASP analysis, and its function was verified via allelic mutants in Chinese cabbage.AbstractBright and glossy green appearance is an attractive commodity character for leafy vegetables and is mainly caused by the absence of epicuticular wax crystals. In this study, two allelic epicuticular wax crystal deficiency mutants,wdm9andwdm10,were obtained from an EMS mutagenesis population of Chinese cabbage (Brassica rapaL. ssp.pekinensis). Genetic analysis revealed that the mutant phenotype was controlled by a recessive nuclear gene.BrKCS6encoding 3-ketoacyl-CoA synthases was identified as the candidate gene by MutMap and KASP analysis. A SNP (G to A) onBrKCS6inwdm9led to the amino acid substitution from serine (S) to phenylalanine (F), and another SNP (G to A) inwdm10resulted in the amino acid substitution from serine (S) to leucine (L). Both SNPs are located in the ACP_syn_III_C conserved domain, corresponding to two highly conserved sites among BrKCS6 and its homologs. These two amino acid substitutions changed the secondary structure and the three-dimensional structure of BrKCS6 protein. qRT-PCR results showed that the relative expression ofBrKCS6significantly decreased in the flower, stem, and leaves in mutant, and the relative expressions of the downstream key genes ofBrKCS6were down-regulated in mutant. We were the first to clone the precious glossy bright geneBrKCS6which has a great potential for commodity quality breeding in Chinese cabbage.