NSOM/QD-based nanoscale immunofluorescence imaging of antigen-specific T-cell receptor responses during an in vivo clonal Vγ2Vδ2 T-cell expansion
NSOM/QD-based nanoscale immunofluorescence imaging of antigen-specific T-cell receptor responses during an in vivo clonal Vγ2Vδ2 T-cell expansion
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DOI：
10.1182/blood-2007-07-101691
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发表时间：
2008-04-15
期刊：
影响因子：
20.3
通讯作者：
Chen, Zheng W.
中科院分区：
文献类型：
--
作者：
Chen, Yong;Shao, Lingyun;Chen, Zheng W.
体内抗原特异性T细胞免疫应答的纳米级成像尚未报道。在这里，结合近场扫描光学显微镜和荧光量子点为基础的纳米技术被用来执行免疫荧光成像的抗原特异性T细胞受体（TCR）的反应在克隆T细胞扩增的体内模型。近场扫描光学显微镜/量子点系统提供了最佳的光学分辨率（
Nanoscale imaging of an in vivo antigen-specific T-cell immune response has not been reported. Here, the combined nearfield scanning optical microscopy- and fluorescent quantum dot-based nanotechnology was used to perform immunofluorescence imaging of antigen-specific T-cell receptor (TCR) response in an in vivo model of clonal T-cell expansion. The near-field scanning optical microscopy/quantum dot system provided a best-optical-resolution (