Putative precursors of vasopressin, oxytocin, and neurophysins in the rat hypothalamus.
Putative precursors of vasopressin, oxytocin, and neurophysins in the rat hypothalamus.
复制标题
大鼠下丘脑中加压素、催产素和神经素的假定前体。
作者：
J. Rosenior;W. North;G. Moore
本文研究了[精氨酸8]加压素（AVP）和催产素（OT）的生物合成，并将所得结果与其相关的神经垂体素（NP）、AVP-NP和OT-NP的研究进行了比较。用SephadexG-75凝胶过滤层析和十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶电泳（PAGE）对酸性条件下的大鼠下丘脑提取液进行了分析。对凝胶色谱流出物的级分和SDS-PAGE凝胶切片的提取物进行RIA，以测定AVP、AVP-NP、OT和OT-NP的免疫反应性前体，使用每种分子的特异性抗血清。凝胶过滤层析和SDS-PAGE中使用氚标记的牛血清白蛋白、卵清蛋白和细胞色素c作为内标。用G-75柱层析法测得AVP前体分子量大于70 K、31 K、13 K、5 K和小于5 K（AVP），用SDS-PAGE法测得AVP前体分子量大于70 K、39 K、15 K和小于5 K（AVP）。用G-75柱层析法测得AVP-NP前体分子量大于70 K、35 K、24 K和12 K（AVP-NP），用SDS-PAGE法测得AVP-NP前体分子量大于19 K和10 K（AVP-NP）。经G-75柱层析和SDS-PAGE分析，OT的分子量分别大于70 K、35 K、19 K、8 K和小于5 K（OT），分别为60 K、35 K、19 K、9 K和小于5 K（OT）。经G-75柱层析鉴定，OT-NP前体分子量大于70 K、17- 18 K和10 K（OT-NP）;经SDS-PAGE鉴定，OT-NP前体分子量大于28 K、18 K和10 K（OT-NP）。这些研究表明，AVP、AVP-NP和OT、OT-NP之间的前体加工存在微小差异，但允许AVP和AVP-NP以及OT和OT-NP存在共同的前体。
The biosynthesis of [arginine8]vasopressin (AVP) and oxytocin (OT) was studied, and the results obtained have been compared with a study of their associated neurophysins (NP), AVP-NP and OT-NP. Rat hypothalamic extracts obtained at acid pH were subjected to Sephadex G-75 gel filtration chromatography and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). Fractions of gel chromatography effluent and extract of SDS-PAGE gel slices were subjected to RIA for immunoreactive precursors of AVP, AVP-NP, OT, and OT-NP using specific antisera fro each molecule. Tritiated bovine serum albumin, ovalbumin, and cytochrome c were used as internal standards during gel filtration chromatography and SDS-PAGE. Molecular weight estimates obtained for precursors of AVP were more than 70K, 31K, 13K, 5K, and less than 5K (AVP) by G-75 chromatography and more than 70K, 39K, 15K, and less than 5K (AVP) by SDS-PAGE. Molecular weight estimates obtained for precursors of AVP-NP were more than 70K, 35K, 24K, and 12K (AVP-NP) by G-75 chromatography and 19K and 10K (AVP-NP) by SDS-PAGE. Molecular weight estimates of OT were more than 70K, 35K, 19K, 8K, and less than 5K (OT) by G-75 chromatography and 60K, 35K, 19K, 9K, and less than 5K (OT) by SDS-PAGE. Precursors of OT-NP were estimated to be more than 70K, 17-18K, and 10K (OT-NP) by G-75 chromatography and 28K, 18K and 10K (Ot-NP) by SDS-PAGE. These studies show that there are small differences in precursor processing among AVP, AVP-NP, and OT, OT-NP, but allow for the existence of common precursors for AVP and AVP-NP and for OT and OT-NP.