Phylogenetic analysis of bacterial community composition in sediment contaminated with multiple heavy metals from the Xiangjiang River in China.
Phylogenetic analysis of bacterial community composition in sediment contaminated with multiple heavy metals from the Xiangjiang River in China.
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DOI：
10.1016/j.marpolbul.2013.02.023
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发表时间：
2013-05
影响因子：
5.8
通讯作者：
Jianyu Zhu;Jing-xia Zhang;Qian Li;Tao Han;Jianping Xie;Yue-hua Hu;L. Chai
中科院分区：
文献类型：
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作者：
Jianyu Zhu;Jing-xia Zhang;Qian Li;Tao Han;Jianping Xie;Yue-hua Hu;L. Chai
了解重金属污染沉积物的生态环境对重金属污染沉积物的生物修复至关重要，在现代工业发展过程中已成为公众关注的问题。为研究湘江重金属污染沉积物的细菌群落组成，选取了4份重金属污染沉积物样品，进行了不依赖培养的分子分析——聚合酶链反应-限制性片段长度多态性（PCR-RFLP）。结果表明，重金属污染对沉积物微生物群落多样性有影响，物种多样性出现在中度污染的沉积物X样品中。这些沉积物中的优势菌科包括α-变形菌门、β-变形菌门和厚壁菌门。α-变形菌群随着重金属含量的增加而显著增加。冗余分析（RDA）也证实了这一现象。
Understanding the ecology of sediments that are contaminated with heavy metals is critical for bioremediating these sediments, which has become a public concern over the course of the development of modern industry. To investigate the bacterial community composition of sediments that are contaminated with heavy metals in the Xiangjiang River, a total of four sediment samples contaminated with multiple heavy metals were obtained, and a culture-independent molecular analysis, polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP), was performed. The results revealed that heavy metal pollution affected the sediment microbial community diversity, and the greatest species diversity appeared in the moderately polluted sediment X sample. The dominant family in these sediments includes α-Proteobacteria, β-Proteobacteria and Firmicutes. Moreover, α-Proteobacteria was significantly increased with increases in heavy metal. A redundancy analysis (RDA) also confirmed this phenomenon.