Genetic recombination of poliovirus in vitro and in vivo:: Temperature-dependent alteration of crossover sites
Genetic recombination of poliovirus in vitro and in vivo:: Temperature-dependent alteration of crossover sites
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DOI：
10.1006/viro.1999.9703
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发表时间：
1999-05-25
期刊：
影响因子：
3.7
通讯作者：
Wimmer, E
中科院分区：
文献类型：
--
作者：
Duggal, R;Wimmer, E
脊髓灰质炎病毒基因组复制过程中发生频率很高的基因重组是一个分子机制知之甚少的过程。在体外无细胞系统中和在体内感染的组织培养细胞中进行的遗传重组研究已经导致了意想不到的观察结果，即温度强烈影响两个重组RNA链之间发生交叉的位点。在体外和体内，在34 ℃下，两条遗传标记的RNA链之间的特异性交换发生在基因组的广泛范围内。相比之下，在37和40摄氏度的体内重组产生的交叉模式，已显着转移到一个区域编码非结构蛋白。通过分析重组体的生长动力学，排除了在37和40 ℃下对重组体的优先选择。在无细胞系统中重组的研究期间，我们发现脊髓灰质炎病毒RNA分子在无细胞系统中复制的能力与其补充由另一种病毒RNA编程的从头病毒合成的能力之间存在直接相关性。(C)北京：科学出版社.
Genetic recombination that occurs with high frequency during poliovirus genome replication is a process whose molecular mechanism is poorly understood. Studies of genetic recombination in a cell-free system in vitro and in infected tissue culture cells in vivo have led to the unexpected observation that temperature strongly influences the loci at which cross-over between the two recombining RNA strands occurs. specifically cross-over between two genetically marked RNA strands in vitro and in vivo at 34 degrees C occurred over a wide range of the genome. In contrast, recombination in vivo at 37 and 40 degrees C yielded cross-over patterns that had shifted dramatically to a region encoding nonstructural proteins. Preferential selection of recombinants at 37 and 40 degrees C was ruled out by analyses of the growth kinetics of the recombinants. During the studies of recombination in the cell-free system we found that there is a direct correlation between the ability of a poliovirus RNA molecule to replicate in the cell-free system and its capacity to complement de novo virus synthesis programmed by another viral RNA. (C) 1999 Academic Press.