Probiotic fortified diet reduces bacterial colonization and translocation in a long-term neonatal rabbit model
Probiotic fortified diet reduces bacterial colonization and translocation in a long-term neonatal rabbit model
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DOI：
10.1016/j.jpedsurg.2009.02.014
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发表时间：
2009-06-01
影响因子：
2.4
通讯作者：
Smith, Samuel D.
中科院分区：
文献类型：
--
作者：
Copeland, Daniel R.;McVay, Marcene R.;Smith, Samuel D.
背景：益生菌强化饮食可减少短期新生兔模型中持续受到病原体攻击时细菌的定植和易位。本研究的目的是确定活益生菌饮食是否能在长期新生兔模型中有效地减少病原体的定植/易位，而不会对胃肠道外的益生菌产生不良影响。方法：通过剖宫产早产1天出生的兔崽被分配到2种饮食中：一种是新配制的饮食（对照组），另一种是添加了活益生菌乳酸乳杆菌的相同饮食。每次饲喂前在两种制剂中添加阴沟肠杆菌。每天灌饲幼犬2次，记录体重。第7天处死家兔，收获器官培养用于靶生物生长。结果：益生菌强化饮食可显著减少肠杆菌向肝脏的易位，并减少其在胃和肺中的定植。没有证据表明乳球菌易位或在胃肠道外定植。结论：在长期的新生儿模型中，该益生菌强化日粮可以有效地减少致病菌的定植和易位，在日粮中添加乳酸乳杆菌可以使益生菌在没有胃肠道外定植或易位的情况下正常生长。(C) 2009爱思唯尔公司版权所有。
Background: Probiotic fortified diet reduces bacterial colonization and translocation in a short-term neonatal rabbit model when continuously challenged with pathogen. The purpose of this study was to determine if live probiotic diet could remain effective at decreasing colonization/translocation of pathogens in a long-term neonatal rabbit model without ill effects of the probiotic outside the gastrointestinal (GI) tract.Methods: Rabbit pups were born via cesarean delivery I day preterm and assigned to 2 diets: a newly formulated diet (controls) vs the same diet fortified with the live probiotic Lactoccocus lactis. Enterobacter cloacae was added to both preparations before each feed. Pups were gavage fed twice daily, and weights were recorded. Rabbits were sacrificed oil day 7, and organs were harvested and cultured for target organism growth.Results: The probiotic fortified diet resulted in a significant decrease in Enterobacter translocation to the liver and decreased colonization in the stomach and lungs. There was no evidence of Lactococccus translocation or colonization Outside of the GI tract. Conclusion: This probiotic fortified diet was effective at decreasing pathogenic bacteria colonization and translocation in a long-term neonatal model, The addition of L lactis to the diet resulted in appropriate growth without any colonization or translocation of the probiotic outside of the GI tract. (C) 2009 Elsevier Inc. All rights reserved.