DE-cadherin, a core component of the adherens junction complex modifies subcellular localization of the Drosophila gap junction protein innexin2
DE-cadherin, a core component of the adherens junction complex modifies subcellular localization of the Drosophila gap junction protein innexin2
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DOI：
10.1080/15419060600631839
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发表时间：
2006-01-01
影响因子：
--
通讯作者：
Hoch, M
中科院分区：
文献类型：
--
作者：
Bauer, R;Weimbs, A;Hoch, M
果蝇缝隙连接编码蛋白多基因家族由8个家族成员组成，它们在上皮细胞形态发生中的作用目前尚不清楚。我们最近发现innexin2在胚胎上皮细胞的组织中起着至关重要的作用。Innexin2蛋白聚集在表皮的顶侧膜域，并与黏附连接的核心蛋白，如DE-钙粘蛋白和Armadillo，β-连环蛋白同源物共定位。在两个突变体中，Innexin2的定位都发生了改变，生化相互作用研究表明，De-cadherin和Armadillo与Innexin2直接相互作用，提示缝隙连接和黏附连接的生物发生之间存在密切联系。我们利用果蝇施耐德细胞组织培养系统进一步研究了innexin2与DE-钙粘蛋白的相互作用。我们的结果提供了证据，证明DE-钙粘附素可能是控制Innexin2向质膜运输和定位的关键成分。
The Drosophila innexin multigene family of gap junction encoding proteins consists of eight family members whose function in epithelial morphogenesis is mostly unknown. We have recently shown that innexin2 plays a crucial role in the organization of embryonic epithelia. Innexin2 protein accumulates in the epidermis in the apico-lateral membrane domain and colocalizes with core proteins of adherens junctions, such as DE-cadherin and Armadillo, the beta-catenin homolog. Innexin2 localization is altered in both armadillo and DE-cadherin mutants Biochemical interaction studies point to a direct interaction of DE-cadherin and Armadillo with innexin2 suggesting a close link between gap junction and adherens junction biogenesis. We have used the Drosophila Schneider cell tissue culture system to further study the interaction of innexin2 with DE-cadherin. Our results provide evidence that DE-cadherin may be a key component to control trafficking, and localization of Innexin2 to the plasma membrane.