Early Gut Colonization of Preterm Infants: Effect of Enteral Feeding Tubes
Early Gut Colonization of Preterm Infants: Effect of Enteral Feeding Tubes
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DOI：
10.1097/mpg.0000000000001104
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发表时间：
2016-06-01
影响因子：
2.9
通讯作者：
Jimenez, Esther
中科院分区：
文献类型：
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作者：
Gomez, Marta;Moles, Laura;Jimenez, Esther
目的：探讨医院内细菌在肠道喂养系统中的潜在定植情况及其对早产儿早期肠道定植的影响。对26例早产儿在出院前不同时间点采集的粪便和胎粪标本进行培养。采用随机扩增多态性DNA和脉冲场凝胶电泳法对牛奶和婴儿粪便样品中的细菌进行鉴定。结果：在物种水平上共鉴定出约4000株细菌。早产儿和奶样粪便中的优势菌为表皮葡萄球菌、S金黄色葡萄球菌、粪肠球菌、粪肠球菌、粘质沙雷氏菌、肺炎克雷伯菌和大肠埃希菌。所有这些物质都在高浓度下存在，与喂养方式无关。随机扩增多态性DNA和脉冲场凝胶电泳技术显示，在两种类型的样品中都发现了几株细菌。此外，扫描电子显微镜显示，在喂养管和管接头的多个部位存在致密的细菌生物膜。结论：新生儿的胃肠道微生物区系与喂养管之间存在细菌菌株的共享。
Objective: The aim of the study was to evaluate the potential colonization of nosocomial bacteria in enteral feeding systems and its effect on early gut colonization of preterm neonates.Methods: Mother's own milk, donor milk, and preterm formula samples obtained after passing through the external part of the enteral feeding tubes were cultured. In addition, meconium and fecal samples from 26 preterm infants collected at different time points until discharge were cultured. Random amplification polymorphism DNA and pulse field gel electrophoresis were performed to confirm the presence of specific bacterial strains in milk and infant fecal samples.Results: Approximately 4000 bacterial isolates were identified at the species level. The dominant species in both feces from preterm infants and milk samples were Staphylococcus epidermidis, S aureus, Enterococcus faecalis, E faecium, Serratia marcescens, Klebsiella pneumoniae, and Escherichia coli. All of them were present at high concentrations independently of the feeding mode. Random amplification polymorphism DNA and pulse field gel electrophoresis techniques showed that several bacteria strains were found in both type of samples. Furthermore, scanning electron microscopy revealed the presence of a dense bacterial biofilm in several parts of the feeding tubes and the tube connectors.Conclusions: There is a sharing of bacterial strains between the neonates' gastrointestinal microbiota and the feeding tubes used to feed them.