Bacterial community associated with Pfiesteria-like dinoflagellate cultures
Bacterial community associated with Pfiesteria-like dinoflagellate cultures
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DOI：
10.1046/j.1462-2920.2001.00207.x
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发表时间：
2001-06-01
影响因子：
5.1
通讯作者：
Belas, R
中科院分区：
文献类型：
--
作者：
Alavi, M;Miller, T;Belas, R
甲藻（真核生物;泡孔动物;甲藻是单细胞真核微生物，与海洋和河口环境中的许多毒性爆发有关。与甲藻群落共存的是细菌群落，它们经历物种组成的变化，竞争营养物质并产生生物活性化合物，包括毒素。作为一项调查的一部分，以了解的作用，在甲藻的生理和寄生虫发生的细菌，我们的特点与实验室培养的四个'Pfiferia-like'甲藻分离自1997年鱼类杀死事件在切萨皮克湾的细菌群落。使用对原核生物16 S rDNA基因序列具有特异性的寡核苷酸引物的聚合酶链反应来表征总细菌群体，包括可培养和不可培养的物种以及可能的内共生细菌。结果表明，甲藻培养物中共存着30多种细菌。甲藻相关细菌的广泛系统发育类型与其他有害藻类相关的细菌类型大体相似，但不完全相同。使甲藻无菌，并将可培养的细菌加回以确定细菌对甲藻生长的贡献。共聚焦扫描激光荧光显微镜与16 S rDNA探针被用来证明一个子集的细菌和甲藻细胞的物理协会。这些数据表明，细菌群落中的一个关键组成部分是海洋α-变形菌群中的物种，与α-3或SAR 83簇关系最密切。
Dinoflagellates (Eukaryota; Alveolata; Dinophyceae) are single-cell eukaryotic microorganisms implicated in many toxic outbreaks in the marine and estuarine environment. Co-existing with dinoflagellate communities are bacterial assemblages that undergo changes in species composition, compete for nutrients and produce bioactive compounds, including toxins. As part of an investigation to understand the role of the bacteria in dinoflagellate physiology and toxigenesis, we have characterized the bacterial community associated with laboratory cultures of four 'Pfiesteria-like' dinoflagellates isolated from 1997 fish killing events in Chesapeake Bay. A polymerase chain reaction with oligonucleotide primers specific to prokaryotic 16S rDNA gene sequences was used to characterize the total bacterial population, including culturable and non-culturable species, as well as possible endosymbiotic bacteria. The results indicate a diverse group of over 30 bacteria species co-existing in the dinoflagellate cultures. The broad phylogenetic types of dinoflagellate-associated bacteria were generally similar, although not identical, to those bacterial types found in association with other harmful algal species. Dinoflagellates were made axenic, and the culturable bacteria were added back to determine the contribution of the bacteria to dinoflagellate growth. Confocal scanning laser fluorescence microscopy with 16S rDNA probes was used to demonstrate a physical association of a subset of the bacteria and the dinoflagellate cells. These data point to a key component in the bacterial community being species in the marine alpha-proteobacteria group, most closely associated with the alpha -3 or SAR83 cluster.