Effects of high-intensity intermittent swimming on PGC-1alpha protein expression in rat skeletal muscle.
Effects of high-intensity intermittent swimming on PGC-1alpha protein expression in rat skeletal muscle.
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DOI：
10.1111/j.1365-201x.2005.01423.x
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发表时间：
2005-05
期刊：
影响因子：
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通讯作者：
S. Terada;K. Kawanaka;M. Goto;T. Shimokawa;I. Tabata
中科院分区：
文献类型：
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作者：
S. Terada;K. Kawanaka;M. Goto;T. Shimokawa;I. Tabata
目的探讨运动强度对大鼠骨骼肌过氧化物酶体增殖物激活受体γ辅激活因子-1 α（PGC-1 α）蛋白表达的影响。方法我们测量了雄性Sprague-Dawley大鼠（年龄：5-6周龄;体重：150-170 g）在单次高强度间歇运动（HIE）或低强度间歇运动（LIE）后骨骼肌中PGC-1 α的含量。在缺氧缺血性脑病期间，大鼠负重（体重的14%）游泳14个20秒的时间段，游泳的时间间隔为10秒的暂停。LIE大鼠游泳无负荷6小时，在两个3小时的会议，分开45分钟的休息。结果HIE后2、6、18 h大鼠上滑车肌PGC-1 α蛋白含量分别较同龄安静对照组增加126、140、126%; LIE后即刻、6和18 h，肌肉中PGC-1 α蛋白含量分别显著升高84%、95%和67%。PGC-1 α蛋白含量在HIE后6 h较LIE后有升高趋势。然而，两个值之间没有统计学显著差异（P = 0.12）。结论无论运动强度如何，当不同刺激方案诱导的刺激达到饱和时，大鼠骨骼肌中PGC-1 α蛋白含量均增加至相当水平。此外，缺氧缺血性脑病是一个强有力的刺激，促进PGC-1 α蛋白的表达，这可能会诱导运动激活的骨骼肌线粒体生物合成。
AIM The aim of the present investigation was to elucidate the effects of exercise intensity on exercise-induced expression of peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha) protein in rat skeletal muscle. METHODS We measured PGC-1alpha content in the skeletal muscles of male Sprague-Dawley rats (age: 5-6 weeks old; body weight: 150-170 g) after a single session of high-intensity intermittent exercise (HIE) or low-intensity prolonged swimming exercise (LIE). During HIE, the rats swam for fourteen 20-s periods carrying a weight (14% of body weight), and the periods of swimming were separated by a 10-s pause. LIE rats swam with no load for 6 h in two 3-h sessions, separated by 45 min of rest. RESULTS After HIE, the PGC-1alpha protein content in rat epitrochlearis muscle had increased by 126, 140 and 126% at 2, 6 and 18 h, respectively, compared with that of the age-matched sedentary control rats' muscle. Immediately, 6 and 18-h after LIE, the PGC-1alpha protein content in the muscle was significantly elevated by 84, 95 and 67% respectively. The PGC-1alpha protein content observed 6 h after HIE tended to be higher than that observed after LIE. However, there was no statistically significant difference between the two values (P = 0.12). CONCLUSION The present investigation suggests that irrespective of the intensity of the exercise, PGC-1alpha protein content in rat skeletal muscle increases to a comparable level when stimuli induced by different protocols are saturated. Further, HIE is a potent stimulus for enhancing the expression of PGC-1alpha protein, which may induce mitochondrial biogenesis in exercise-activated skeletal muscle.