Analysis of free and esterified sterols in fats and oils by flash chromatography, gas chromatography and electrospray tandem mass spectrometry
Analysis of free and esterified sterols in fats and oils by flash chromatography, gas chromatography and electrospray tandem mass spectrometry
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DOI：
10.1016/s0021-9673(01)01315-2
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发表时间：
2001-11-23
影响因子：
4.1
通讯作者：
Kuuranne, T
中科院分区：
文献类型：
--
作者：
Kalo, P;Kuuranne, T
建立了一种分析游离甾醇和酯化甾醇的方法。将脂肪或油样品在固相萃取硅胶柱上分离成甾醇酯馏分、三酰基甘油馏分和含有部分酰基甘油和残余三酰基甘油的游离甾醇馏分。游离甾醇级分的甾醇酯和酰基甘油被酯交换成甲酯。用气相色谱法测定了甾醇酯馏分中的脂肪酸甲酯、甾醇酯馏分和游离甾醇馏分中的游离甾醇。记录甾醇酯馏分中甾醇碎片离子的前体离子电喷雾MS-MS，并用于测定乳脂和植物油样品中甾醇酯的比例。(C)2001 Elsevier Science B. V.保留所有权利。
A method for the analysis of free and esterified sterols has been developed. Fat or oil samples were separated on solid-phase extraction silica gel columns into a sterol ester fraction, a fraction of triacylglycerols, and a free sterol fraction containing partial acylglycerols and residual triacylglycerols. Sterol esters and acylglycerols of the free sterol fraction were transesterified to methyl esters. The fatty acid methyl esters front sterol ester fraction and the free sterols from sterol ester fraction and free sterol fraction were determined by GLC. Precursor ion electrospray MS-MS of sterol fragment ions of sterol ester fractions were recorded and used for determination of sterol ester proportions in butterfat and vegetable oil samples. (C) 2001 Elsevier Science B.V. All rights reserved.