LOCALIZATION OF ORIGINS OF ELECTRORETINOGRAM COMPONENTS BY INTRARETINAL RECORDING IN INTACT CAT EYE
LOCALIZATION OF ORIGINS OF ELECTRORETINOGRAM COMPONENTS BY INTRARETINAL RECORDING IN INTACT CAT EYE
复制标题
DOI：
10.1113/jphysiol.1961.sp006768
复制
发表时间：
1961-01-01
影响因子：
5.5
通讯作者：
WIESEL, TN
中科院分区：
文献类型：
--
作者：
BROWN, KT;WIESEL, TN
暗适应猫的4个主要成分是a波、B波、c波和直流波。当地的尔格。由视网膜内导联记录的LERG也表现出这些成分，并且仅用于本工作。a波、B波和c波的振幅以及直流分量被绘制为电极深度的函数。a波和c波的最大振幅紧邻Bruch膜的视网膜侧，而b波和d.c.分量的最大振幅位于内核层外缘的稍远侧（脉络膜）。利多卡因（利多卡因）完全、选择性和可逆地消除了b波。因此，b波取决于传导脉冲。由于唯一明显远离b波起源的结构是感受器，这也表明感受器纤维传导冲动。虽然直流成分似乎起源于与B波相同的视网膜水平，但它不受利多卡因的影响，似乎只依赖于电紧张传导活动。利多卡因未能降低a波或c波与这些成分来源的其他证据一致。罕见的细胞内记录是在非常接近布鲁赫膜的视网膜侧获得的，据信是来自色素上皮的单细胞。这些记录显示，光反应的时间过程与c波相同，但极性相反，幅度更大。因此，它们似乎是来自产生c波的细胞的细胞内记录。细胞内的记录也获得了从单个细胞只是远端的内核层，光响应慢电位。反应包含快速和直流相，其时间过程分别与lerg的b波和直流分量相同。由于这些细胞内记录在相同的视网膜水平，其中b波和直流分量的振幅最大，这表明b波和直流分量都是由这些细胞产生的。这篇论文的发现，结合其他几条证据，指出a波的起源在受体的外段。
The 4 major components of the dark-adapted cat erg are the a-, b-, and c-waves plus a d.c. component. The local erg. (lerg) recorded by an intraretinal lead also exhibits these components and was used exclusively in the present work. The amplitude of a-, b-, and c-waves and a d.c. component were plotted as a function of electrode depth. The a- and c-waves had maximum amplitudes immediately adjacent to the retinal side of Bruch''s membrane, while the b-wave and d.c. component had amplitude maxima slight distal (choroidal) to the outer margin of the inner nuclear layer. Xylocaine (lignocaine) abolished the b-wave completely, selectively, and reversibly. The b-wave therefore depends upon conducted impulses. Since the only structures clearly distal to the origin of the b-wave are the receptors, this also indicates that receptor fibres conduct impulses. Although the d.c. component seems to originate at the same retinal level as the b-wave, it was unaffected by xylocaine and appears to depend only upon electrotonically conducted activity. Failure of xylocaine to reduce the a- or c-wave agrees with other evidence on the origins of these components. Rare intracellular recordings were obtained very close to the retinal side of Bruch''s membrane and were believed to be derived from single cells of the pigment epithelium. These records showed light responses with time course identical to that of the c-wave, but opposite in polarity and much larger in magnitude. Hence they appeared to be intracellular records from the cells which produce the c-wave. Intracellular records were also obtained from single cells just distal to the inner nuclear layer which responded to light only with slow potentials. The response contained both a rapid and a d.c. phase, and the time courses of these appeared identical with the b-wave and d.c. component, respectively, of the lerg. Since these intracellular records were at the same retinal level where the b-wave and d.c. components were maximum in amplitude, it is suggested that the b-wave and d.c. component are both generated by these cells. The findings of this paper, in conjunction with several other lines of evidence, point to an origin of the a-wave in the outer segments of receptors.