Expression and distribution of leptospiral outer membrane components during renal infection of hamsters
Expression and distribution of leptospiral outer membrane components during renal infection of hamsters
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DOI：
10.1128/iai.67.2.853-861.1999
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发表时间：
1999-02-01
影响因子：
3.1
通讯作者：
Haake, DA
中科院分区：
文献类型：
--
作者：
Barnett, JK;Barnett, D;Haake, DA
致病性钩端螺旋体在培养基中的外膜含有脂多糖（LPS）、一种孔蛋白（OmpL1）和几种脂蛋白，包括LipL36和LipL41。本研究的目的是表征这些外膜抗原在肾脏感染期间的表达和分布。用宿主源性克氏钩端螺旋体刺激仓鼠产生含有体内表达抗原抗体的血清。用这些宿主来源的生物体攻击动物的血清进行免疫印迹分析，结果显示与OmpL1、LipL41和其他几种蛋白质有反应性，但与LipL36没有反应性。虽然LipL36是培养的克氏乳杆菌的重要外膜抗原，但免疫组化也无法在感染的仓鼠肾组织中检测到其表达，表明该蛋白在体内表达下调。相比之下，LPS、OmpL1和LipL41在感染后10天和28天被证实在近曲肾小管管腔中定植。管腔集落周围的管状上皮细胞有细颗粒状的胞浆LPS。感染后28天，当肾间质出现细胞炎症反应时，间质吞噬细胞中也可检测到LPS和OmpL1。这些数据表明，感染期间表达的外膜成分在钩端螺旋体间质性肾炎的诱导和持续中起作用。
The outer membrane of pathogenic Leptospira species grown in culture media contains lipopolysaccharide (LPS), a porin (OmpL1), and several lipoproteins, including LipL36 and LipL41. The purpose of this study was to characterize the expression and distribution of these outer membrane antigens during renal infection. Hamsters were challenged with host-derived Leptospira kirschneri to generate sera which contained antibodies to antigens expressed in vivo. Immunoblotting performed with sera from animals challenged with these host-derived organisms demonstrated reactivity with OmpL1, LipL41, and several other proteins but not with LipL36. Although LipL36 is a prominent outer membrane antigen of cultivated L. kirschneri, its expression also could not be detected in infected hamster kidney tissue by immunohistochemistry, indicating that expression of this protein is down-regulated in vivo. In contrast, LPS, OmpL1, and LipL41 were demonstrated on organisms colonizing the lumen of proximal convoluted renal tubules at both 10 and 28 days postinfection. Tubular epithelial cells around the luminal colonies had fine granular cytoplasmic LPS. When the cellular inflammatory response was present in the renal interstitium at 28 days postinfection, LPS and OmpL1 were also detectable within interstitial phagocytes. These data establish that outer membrane components expressed during infection have roles in the induction and persistence of leptospiral interstitial nephritis.