Water tracing with environmental DNA in a high-Alpine catchment
Water tracing with environmental DNA in a high-Alpine catchment
复制标题
利用环境 DNA 对高山流域的水进行追踪
DOI：
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
N. Ceperley
中科院分区：
文献类型：
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作者：
E. Mächler;Anham Salyani;J. Walser;A. Larsen;B. Schaefli;F. Altermatt;N. Ceperley
抽象的。高山溪流对于下游水资源和生态系统保护特别有价值。然而，在异质的山区环境中，水在何时何地储存和释放的细节却鲜为人知。由于水以雪和冰的形式暂时积累和储存，物理化学流路示踪剂的使用特别具有挑战性。另外，生物示踪剂可以补充有关水流和储存的信息，特别是当高山水生系统的不同微生境居住着特征性的生物群落时。在这项研究中，我们探索了水中发现的环境 DNA 颗粒 (eDNA) 在表征瑞士阿尔卑斯流域的水文流动路径和连通性方面的潜力。 2017 年 3 月至 9 月期间，我们在分布于 13.4 平方公里 Vallon de Nant 流域的 11 个地点的多个时间点进行了水采样，以获取基于自然发生的 eDNA 的遗传物种信息。这些地点对应于三种不同的水源类型和栖息地（主河道、支流和泉水）。典型水文示踪剂和 eDNA 与水流时间演化的比较表明，在主河道和支流中，水流的变化 dq/dt 与生物丰富度密切相关。在泉水中，电导率被发现与生物丰富度呈正相关，但相关性不那么强。在流域范围内，我们的结果表明，eDNA 样本检测到的多样性表明了生物丰富度。当水流增加时，额外的（可能是陆地的）DNA 会被输送到储水室或流动室中。这些过程包括溢流、河流网络扩展和收缩以及潜流交换。总的来说，我们的结果强调了考虑现场采样栖息地与上游相连栖息地的重要性，以了解溪流如何在流域内整合 eDNA 并解释空间分布的 eDNA 样本，以进行水文和生物多样性评估。我们确定了使用 eDNA 作为高山水源的独立示踪剂的后续步骤，并为未来在高山溪流生态系统中观察 eDNA 提供了建议。
Abstract. Alpine streams are particularly valuable for downstream water resources and for ecosystem conservation. However, the details of where and when water is stored and released in the heterogeneous mountain environment are rarely known. The use of physico-chemical flow path tracers is particularly challenging due to the temporary accumulation and storage of water in the form of snow and ice. Alternatively, biological tracers might complement information on flow and storage of water, especially as the different microhabitats in Alpine aquatic systems are inhabited by characteristic organismal communities. In this study, we explored the potential of particles of environmental DNA found in the water (eDNA) to characterize hydrological flow paths and connectivity in an Alpine catchment in Switzerland. Between March and September 2017, we sampled water at multiple time points at 11 sites distributed over the 13.4 km2 Vallon de Nant catchment for genetic species information based on naturally occurring eDNA. The sites correspond to three different water source types and habitats (main channel, tributaries, and springs). Comparison of typical hydrological tracers and eDNA with temporal evolution of streamflow revealed that in the main channel and in the tributaries, the change in streamflow, dq/dt, is strongly correlated with biological richness. In springs, electrical conductivity was found to have a positive but not as strong correlation with biological richness. At the catchment scale, our results show that biological richness as indicated by the diversity detected by eDNA samples. When streamflow is increasing, transport of additional, and probably terrestrial, DNA into water storage or flow compartments is occurring. Such processes include overbank flow, stream network expansion and retraction, and hyporheic exchange. In general, our results highlight the importance of considering the at-site sampling habitat in combination with upstream connected habitats to understand how streams integrate eDNA over a catchment and to interpret spatially distributed eDNA samples, both for hydrological and biodiversity assessments. We identify next steps to be addressed to use eDNA as an independent tracer of Alpine water sources and we provide recommendations for future observation of eDNA in Alpine stream ecosystems.
影响因子：
30.7
作者：
Jacobsen, Dean;Milner, Alexander M.;Dangles, Olivier
通讯作者：
Dangles, Olivier