Characterization of the Saccharomyces cerevisiae high affinity copper transporter Ctr3
Characterization of the Saccharomyces cerevisiae high affinity copper transporter Ctr3
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DOI：
10.1074/jbc.m005392200
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发表时间：
2000-10-27
影响因子：
4.8
通讯作者：
Thiele, DJ
中科院分区：
文献类型：
--
作者：
Peña, MMO;Puig, S;Thiele, DJ
铜是许多具有不同生物学作用的酶的活性所需的必需营养素，在面包酵母酿酒酵母中，铜通过两种高亲和力的铜转运蛋白Ctr 1和Ctr 3转运到细胞中。尽管Ctr1和Ctr3在功能上是冗余的，但它们在氨基酸序列水平上几乎没有同源性。在这份报告中，我描述了Ctr3的定位、组装和转录后调控。Ctr3是一种整合的膜蛋白，其组装成三聚体以在质膜上形成感受态铜摄取通透酶，而CTR 1和CTR 3基因在转录水平上响应于铜而类似地受到调节，这些蛋白质的转录后调节是不同的，与Ctr1不同，Ctr3转运蛋白既不受蛋白质降解水平的调节，也不受作为升高的铜水平的函数的内吞作用的调节。我们的研究表明，Ctr3构成了一个基本的模块，发现在所有真核生物的高亲和力铜转运，这是足够的铜摄取，但缺乏铜的转录后调控的元素。
Copper is an essential nutrient required for the activity of a number of enzymes with diverse biological roles, In the bakers' yeast Saccharomyces cerevisiae, copper is transported into cells by two high affinity copper transport proteins, Ctr1 and Ctr3. Although Ctr1 and Ctr3 are functionally redundant, they bear little homology at the amino acid sequence level, In this report, me characterize Ctr3 with respect to its localization, assembly, and post-transcriptional regulation. Ctr3 is an integral membrane protein that assembles as a trimer to form a competent copper uptake permease at the plasma membrane, Whereas the CTR1 and CTR3 genes are similarly regulated at the transcriptional level in response to copper, post-transcriptional regulation of these proteins is distinct, Unlike Ctr1, the Ctr3 transporter is neither regulated at the level of protein degradation nor endocytosis as a function of elevated copper levels. Our studies suggest that Ctr3 constitutes a fundamental module found in all eukaryotic high affinity copper transporters to date, which is sufficient for copper uptake but lacks elements for post-transcriptional regulation by copper.