Methanol oxidation mutants in Methylobacterium extorquens AM1: identification of new genetic complementation groups.
Methanol oxidation mutants in Methylobacterium extorquens AM1: identification of new genetic complementation groups.
复制标题
扭扭甲基杆菌 AM1 中的甲醇氧化突变体：新遗传互补组的鉴定。
DOI：
10.1099/13500872-141-11-2985
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Lidstrom,ME
中科院分区：
文献类型：
--
作者：
Springer,AL;Chou,HH;Fan,WH;Lee,E;Lidstrom,ME
分离出 28 个新的扭动甲基杆菌 AM1 甲醇氧化 (Mox) 突变体并放入互补组中。互补分析确定了 Mxb 和 Mxc 位点以及新位点 Mxd 中的新 Mox 基团。 Mxb 位点的 37 个突变体根据其互补模式分为 MxbM 和 MxbD 互补组。 Mxc 基因座的 29 个突变体分为三个互补组：MxcB、MxcQ 和 MxcE。该基因座的基因转录方向可以从亚克隆中推断出来。十八个新突变体没有被先前分离的 M 所补充。 extorquensAM1 克隆，但由两个新的重叠克隆补充。该基因座称为 Mxd，突变体分为两个互补组：MxdR 和 MxdS。所有这些突变类别的免疫印迹显示，与野生型相比，所有 Mxb 和 Mxc 菌株的 MxaF（甲醇脱氢酶大亚基）和细胞色素cL 水平均显着降低。这些突变体，特别是 Mxb 突变体，也具有升高的 cytochromac-553 水平。这些结果与MxbMD和MxcBQE互补基团在mxaF表达调节中的作用一致。 MxdR和MxdS突变体具有正常水平的MxaF和bothc型细胞色素。
Two-hundred-and-eight newMethylobacterium extorquensAM1 methanol oxidation (Mox) mutants were isolated and placed into complementation groups. Complementation analyses identified new Mox groups in the Mxb and Mxc loci and at a new locus, Mxd. Thirty-seven mutants at the Mxb locus were divided into MxbM and MxbD complementation groups on the basis of their complementation pattern. Twenty-nine mutants at the Mxc locus fell into three complementation groups, MxcB, MxcQ and MxcE. The direction of transcription for genes at this locus could be inferred from the subclones. Eighteen of the new mutants were not complemented by previously isolatedM. extorquensAM1 clones but were complemented by two new overlapping clones. This locus was called Mxd and the mutants fell into two complementation groups, MxdR and MxdS. Immunoblots from all these mutant classes showed that all of the Mxb and Mxc strains had substantially reduced levels of MxaF (large subunit of methanol dehydrogenase) and cytochromecL, compared to the wild-type. These mutants, particularly the Mxb mutants, also had elevated levels of cytochromac-553. These results are consistent with a role for the MxbMD and MxcBQE complementation groups in the regulation of expression ofmxaF. The MxdR and MxdS mutants had normal levels of MxaF and bothc-type cytochromes.