In vitro evaluation of the antimicrobial activity of five root canal sealers
In vitro evaluation of the antimicrobial activity of five root canal sealers
复制标题
DOI：
10.1590/s0103-64402004000100006
复制
发表时间：
2004-01-01
影响因子：
--
通讯作者：
Souza-Filho, Francisco José de
中科院分区：
文献类型：
--
作者：
Gomes, Brenda Paula Figueiredo de Almeida;Pedroso, José Assis;Souza-Filho, Francisco José de
本研究的目的是分析五种牙髓封闭剂的抗菌特性：Endo Fill、Endomethasone、Endomethasone N、Sealer 26 和 AH-Plus，针对以下微生物：白色念珠菌、金黄色葡萄球菌、粪肠球菌、血链球菌和内氏放线菌。封闭剂在操作后立即、24小时、48小时和7天进行测试。通过直接接触法观察液体培养基中微生物的生长情况和琼脂扩散试验来评价封闭剂的抗菌性能。使用两种方法的结果表明，在操作后立即，Endo-Fill 和 Endomethasone 表现出最高的抗菌活性，并且它们之间没有统计学上的显着差异。 Sealer 26 的抗菌活性最低。在操作后的所有其他时间，所有测试的封闭剂之间没有统计学上的显着差异。总之，没有一种封闭剂能够完全抑制微生物的生长。此外，每种封闭剂的抗菌活性随着时间的推移而降低，并且取决于微生物对其的敏感性。
The aim of the present study was to analyze the antimicrobial properties of five endodontic sealers: Endo Fill, Endomethasone, Endomethasone N, Sealer 26 and AH-Plus, against the following microorganisms: Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus sanguis and Actinomyces naeslundii. The sealers were tested immediately, 24 h, 48 h and 7 days after manipulation.The direct contact method through the observation of the microbial growth in liquid medium and the agar diffusion test were used to evaluate the antimicrobial properties of the sealers. The results, in both methodologies used, showed that immediately after manipulation, Endo-Fill and Endomethasone demonstrated the highest antimicrobial activity, with no statistically significant difference between them. Sealer 26 demonstrated the lowest antimicrobial activity. At all other times after manipulation, there were no statistically significant differences among all the sealers tested. In conclusion, none of the sealers totally inhibited the growth of the microorganisms. Furthermore, the antimicrobial activity of each sealer decreased with time and was dependent upon the microbial susceptibility to them.