Comparison of Hepatitis C Virus RNA and antibody detection in dried blood spots and plasma specimens
Comparison of Hepatitis C Virus RNA and antibody detection in dried blood spots and plasma specimens
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DOI：
10.1016/j.jcv.2014.01.014
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发表时间：
2014-04-01
影响因子：
8.8
通讯作者：
Page, Kimberly
中科院分区：
文献类型：
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作者：
Dokubo, E. Kainne;Evans, Jennifer;Page, Kimberly
背景资料：目前丙型肝炎病毒（HCV）的诊断检测包括抽血和HCV抗体（抗HCV）和RNA的血清学检测，这些检测并不总是可行的。干血斑（DBS）是一种微创的采样方法，适用于样本采集、储存和检测。目的：为了评估DBS在HCV检测中的实用性，我们评估了DBS与血浆标本相比检测抗HCV和HCV RNA的灵敏度和特异性。这项横断面验证研究是在现有的年轻注射吸毒者中HCV前瞻性研究的背景下进行的。通过静脉穿刺将血液样品收集到血清分离管（SST）中，并通过手指针刺到Whatman 903（R）蛋白质保存卡上。使用第三代酶联或荧光免疫测定法（IA）检测血浆样本和DBS洗脱液中的抗HCV，并使用区分性HCV转录介导扩增试验（dHCV TMA）检测HCV RNA。DBS结果进行了比较，他们相应的血浆样本results.Results：148名参与者进行了抗-HCV和132名参与者进行了HCV RNA检测。DBS检测抗-HCV的敏感性为70%，特异性为100%，阳性预测值（PPV）为100%，阴性预测值（NPV）为76%，Kappa值为0.69。DBS检测HCVRNA的敏感性为90%，特异性为100%，PPV为100%，NPV为94%，Kappa为0.92。结论：DBS检测抗-HCV和HCVRNA敏感性高，特异性强，与血浆检测结果相关性好，对HCV感染的诊断有一定的参考价值。(C)2014爱思唯尔有限公司版权所有。
Background: Current diagnostic tests for Hepatitis C Virus(HCV) involve phlebotomy and serologic testing for HCV antibodies (anti-HCV) and RNA, which are not always feasible. Dried blood spots (DBS) present a minimally invasive sampling method and are suitable for sample collection, storage and testing.Objectives: To assess the utility of DBS in HCV detection, we evaluated the sensitivity and specificity of DBS for anti-HCV and HCV RNA detection compared to plasma specimens.Study design: This cross-sectional validation study was conducted in the context of an existing prospective study of HCV in young injection drug users. Blood samples were collected by venipuncture into serum separator tubes (SST) and via finger stick onto Whatman 903 (R) protein-saver cards. Plasma samples and eluates from the DBS were tested for anti-HCV using either a third generation enzyme-linked or chemiluminescent immunoassay (IA), and HCV RNA using discriminatory HCV transcription-mediated amplification assay (dHCV TMA). DBS results were compared to their corresponding plasma sample results.Results: 148 participants were tested for anti-HCV and 132 participants were tested for HCV RNA. For anti-HCV, the sensitivity of DBS was 70%, specificity was 100%, positive predictive value (PPV) was 100%, negative predictive value (NPV) was 76% and Kappa was 0.69. For HCV RNA, the sensitivity of DBS was 90%, specificity was 100%, PPV was 100%, NPV was 94% and Kappa was 0.92.Conclusions: DBS are sensitive and very specific in detecting anti-HCV and HCV RNA, demonstrate good correlation with plasma results, and have potential to facilitate diagnosis of HCV infection. (C) 2014 Elsevier B.V. All rights reserved.