Aberrations of the tumor suppressor p53 and retinoblastoma genes in human hepatocellular carcinomas.
Aberrations of the tumor suppressor p53 and retinoblastoma genes in human hepatocellular carcinomas.
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发表时间：
1991-10
期刊：
影响因子：
11.2
通讯作者：
Y. Murakami;Kenshi Hayashi;S. Hirohashi;T. Sekiya
中科院分区：
文献类型：
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作者：
Y. Murakami;Kenshi Hayashi;S. Hirohashi;T. Sekiya
应用聚合酶链反应（PCR）产物单链构象多态性（SSCP）分析方法，检测了43例原发性肝细胞癌（HCC）中p53基因的异常。在这些肝细胞癌中，22例为晚期HCC，21例为早期HCC。p53基因结构异常在22例晚期肝癌中的8例中观察到，但在早期肝癌中没有发现。在8例p53基因异常的肿瘤中，7例丢失了两个p53等位基因中的一个，在7例具有可识别突变的肿瘤中，在4例肿瘤中发现了点突变，在2例肿瘤中观察到了几个核苷酸的缺失。剩下的一个保留了两个等位基因，并携带两个点突变。除了p53基因的畸变，视网膜母细胞瘤基因的丢失或染色体13 q杂合性丢失，观察到在7个信息的情况下，8个肿瘤携带突变的p53基因。这些结果表明，在肝癌发生的晚期阶段，至少有两个肿瘤抑制基因的参与。
Aberrations of the p53 gene in 43 primary hepatocellular carcinomas (HCCs) were examined by single-strand conformation polymorphism analysis of polymerase chain reaction products. Of these hepatocellular carcinomas, 22 were advanced HCCs, and 21 were early HCCs. Structural abnormalities of the p53 gene were observed in eight of the 22 advanced HCCs, but in none of the early HCCs. Of the eight tumors with an abnormal p53 gene, seven had lost one of the two p53 alleles and, in the seven tumors with identifiable mutations, point mutations were found in four tumors and deletions of several nucleotides were observed in two tumors. The remaining one retained both alleles and carried two point mutations. In addition to the aberrations of the p53 gene, loss of the retinoblastoma gene or loss of heterozygosity at chromosome 13q was observed in six of seven informative cases of eight tumors carrying a mutated p53 gene. These results suggest the involvement of at least two tumor suppressor genes in a late stage of hepatocarcinogenesis.