Novel disintegrin-like peptides derived from an amphibian skin cDNA sequence of Hypsiboas punctatus.
Novel disintegrin-like peptides derived from an amphibian skin cDNA sequence of Hypsiboas punctatus.
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源自 Hypsiboas punctatus 两栖动物皮肤 cDNA 序列的新型解整合素样肽。
DOI：
10.1002/psc.3382
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
BlochJr,Carlos
中科院分区：
文献类型：
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作者：
Pires,DiegoAT;Tacca,LuisaMRA;Aslan,JosephE;Murad,AndréM;Nascimento,ClaudiaJ;Barbosa,EderA;BlochJr,Carlos
解整合素由一系列小蛋白组成，它们结合并改变整合素的生理功能，特别是介导血液中血小板聚集的整合素。在这里，我们报道了两栖动物 Hypsiboas punctatusskin 的 cDNA 文库中鉴定出的 15 个氨基酸残基肽中存在的赖氨酸-甘氨酸-天冬氨酸 (KGD) 解整合素样基序。以原始肽序列为模板，设计了五种新的类似物，通过固相化学合成，并使用核磁共振波谱测试了解整合素活性和三维结构研究。原始的两栖动物肽对整合素介导的反应没有影响。然而，衍生肽类似物抑制整合素介导的血小板功能，包括血小板在纤维蛋白原上的扩散。
Disintegrins comprise a family of small proteins that bind to and alter the physiological function of integrins, especially integrins that mediate platelet aggregation in blood. Here, we report a lysine‐glycine‐aspartic acid (KGD) disintegrin‐like motif present in a 15‐amino acid residue peptide identified in a cDNA library of the amphibianHypsiboas punctatusskin. The original peptide sequence was used as a template from which five new analogs were designed, chemically synthesized by solid phase, and tested for disintegrin activity and tridimensional structural studies using NMR spectroscopy. The original amphibian peptide had no effect on integrin‐mediated responses. Nevertheless, derived peptide analogs inhibited integrin‐mediated platelet function, including platelet spreading on fibrinogen.