ROLE OF SUPEROXIDE RADICAL IN A NONENZYMATIC HYDROXYLATION
ROLE OF SUPEROXIDE RADICAL IN A NONENZYMATIC HYDROXYLATION
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DOI：
10.1016/0003-9861(72)90398-0
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发表时间：
1972-01-01
影响因子：
3.9
通讯作者：
FRIDOVICH, I
中科院分区：
文献类型：
--
作者：
GOSCIN, SA;FRIDOVICH, I
在含有二羟基富马酸、FeSO 4和O2的反应混合物中，对甲酚和4-硝基苯酚被羟基化。羟基化产物的积累表现出初始滞后，而二羟基富马酸的氧化没有。这种滞后被H2 O2消除。羟基化被部分抑制超氧化物歧化酶，过氧化氢酶，或甲酸和完全抑制乙醇。结果表明，活性羟基化物种是OH·，而O2−虽然不直接作为羟基化剂发挥作用，但确实通过涉及H2 O2的反应产生OH·。
P-Cresol and 4-nitrophenol were hydroxylated in reaction mixtures containing dihydroxyfumaric acid, FeSO4and O2. The accumulation of hydroxylated product exhibited an initial lag whereas the oxidation of dihydroxyfumaric acid did not. This lag was eliminated by H2O2. Hydroxylation was partially inhibited by Superoxide dismutase, catalase, or formate and was completely inhibited by ethanol. The results suggest that the active hydroxylating species was OH· and that O2−, although not functioning directly as a hydroxylating agent, did serve to generate OH· by a reaction which involved H2O2.