Coexistence and gene expression of phenylethanolamine N-methyltransferase, tyrosine hydroxylase, and neuropeptide tyrosine in the rat and bovine adrenal gland: effects of reserpine.
Coexistence and gene expression of phenylethanolamine N-methyltransferase, tyrosine hydroxylase, and neuropeptide tyrosine in the rat and bovine adrenal gland: effects of reserpine.
复制标题
大鼠和牛肾上腺中苯乙醇胺 N-甲基转移酶、酪氨酸羟化酶和神经肽酪氨酸的共存和基因表达：利血平的影响。
DOI：
10.1073/pnas.85.21.8306
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发表时间：
1988
影响因子：
11.1
通讯作者：
Hökfelt,T
中科院分区：
文献类型：
--
作者：
Schalling,M;Dagerlind,A;Brené,S;Hallman,H;Djurfeldt,M;Persson,H;Terenius,L;Goldstein,M;Schlesinger,D;Hökfelt,T
儿茶酚胺合成酶苯乙醇胺n -甲基转移酶（PNMTase； s -腺苷- l-蛋氨酸：苯乙醇胺n -甲基转移酶，EC 2.1.1.28）和酪氨酸羟化酶[TyrOHase；研究了大鼠和牛肾上腺髓质中酪氨酸3-单加氧酶、l -酪氨酸、四氢蝶啶氧氧化还原酶（3-羟基化）、EC 1.14.16.2和共存的神经肽酪氨酸（NPY）。通过免疫组织化学和原位杂交，PNMTase阳性细胞和npy阳性细胞在牛髓质中表现出紧密的重叠，并优先定位于外三分之二的髓质。虽然TyrOHase及其mRNA在几乎所有的髓样腺细胞中都存在，但在PNMTase和npy阳性细胞中，TyrOHase mRNA水平要高得多。大鼠给予儿茶酚胺消耗药物利血平后，观察到肾上腺髓质PNMTase mRNA水平短暂增加，然后急剧下降。相比之下，TyrOHase和NPY的mRNA均表现出增加，TyrOHase的mRNA峰值先于NPY的mRNA峰值。因此，这三种化合物在肾上腺髓质中共存可能存在不同的调节机制。
Expression and regulation of the catecholamine-synthesizing enzymes phenylethanolamine N-methyltransferase (PNMTase; S-adenosyl-L-methionine:phenylethanolamine N-methyltransferase, EC 2.1.1.28) and tyrosine hydroxylase [TyrOHase; tyrosine 3-monooxygenase, L-tyrosine, tetrahydropteridine:oxygen oxidoreductase (3-hydroxylating), EC 1.14.16.2] and the coexisting neuropeptide tyrosine (NPY) were studied in rat and bovine adrenal medulla. By using both immunohistochemistry and in situ hybridization, PNMTase- and NPY-positive cells exhibited a close overlap in bovine medulla and were preferentially localized in the outer two-thirds of the medulla. Although TyrOHase and its mRNA were observed in virtually all medullary gland cells, TyrOHase mRNA levels were much higher in the PNMTase- and NPY-positive cells. After administration of the catecholamine-depleting drug reserpine to rats, a brief increase, followed by a dramatic decrease, in the level of PNMTase mRNA was observed in the adrenal medulla. In contrast, mRNA for both TyrOHase and NPY only exhibited an increase, whereby the TyrOHase mRNA peak preceded that of NPY mRNA. Different regulatory mechanisms may thus operate for these three compounds coexisting in the adrenal medulla.