Assessment of the aetiology of acute gastroenteritis outbreaks in infants reveals rotavirus, noroviruses and adenovirus prevalence and viral coinfections in Nsukka, Nigeria.
Assessment of the aetiology of acute gastroenteritis outbreaks in infants reveals rotavirus, noroviruses and adenovirus prevalence and viral coinfections in Nsukka, Nigeria.
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对婴儿急性胃肠炎暴发的病因学评估显示，尼日利亚恩苏卡存在轮状病毒、诺如病毒和腺病毒流行以及病毒合并感染。
DOI：
10.1007/s13337-023-00821-2
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Chigor VN
中科院分区：
文献类型：
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作者：
Chigor VN
更好地了解尼日利亚东南部急性胃肠炎（AGE）暴发的病因将有助于保障公众健康。这项研究对恩苏卡选定医院的婴儿（<5 岁儿童）收集的粪便样本进行了人类肠道病毒筛查，并根据选定医院的三年记录评估了 AGE 的季节性。在 2019 年 1 月至 3 月和 2020 年 1 月至 2 月的 AGE 爆发期间收集了总共 120 份粪便样本（109 份来自腹泻患者，11 份来自非腹泻患者，作为对照）。使用免疫层析侧向层析检测对样本进行分析，对轮状病毒 (RoV)、腺病毒 (AdV) 和诺如病毒基因组 I 和 II (NoVI、第七）。还收集并分析了医院报告的 AGE 病例的三年（2017-2019）回顾性数据。急性胃肠炎总体患病率较高（75.83%），其中病毒合并感染占13.19%。轮状病毒检出率（69.17%）高于其他病毒制剂（15.83%）。 RoV、AdV 和 NoVII 均观察到单一感染和混合感染，而 NoVI 仅在混合感染病例中检测到。危险因素分析显示，1岁婴儿（73.53%）急性胃肠炎的发病率高于1≤≤2岁（22.55%）或≥2岁（3.92%）的婴儿。性别和年龄与合并感染病例无关（p˂0.05）。季节性数据显示，2017年1月出现感染高峰，随后两年连续下降。这些结果证明了恩苏卡婴儿腹泻病例中肠道病毒的流行和共存。该地区肠道病毒株（尤其是诺如病毒）的进一步分子特征将对全球流行病学数据做出重大贡献。
A better understanding of the aetiology of acute gastroenteritis (AGE) outbreaks in Southeast Nigeria would help safeguarding public health. This study screened stool samples collected from infants (children < 5 years of age) attending selected hospitals in Nsukka for human enteric viruses and evaluated the seasonality of AGE based on three-year records available at selected hospitals. A total of 120 stool samples (109 from diarrhoeal-patients and 11 from non-diarrhoeal patients, as control) collected during the AGE outbreaks of January – March 2019 and January–February 2020. The samples were analysed using an immunochromatographic lateral flow assay for differential qualitative detection of rotavirus (RoV), adenovirus (AdV), and norovirus genogroups I and II (NoVI, NoVII). Three-year (2017–2019) retrospective data on the cases of AGE reported at the hospitals were also collected and analysed. The overall prevalence of acute gastroenteritis was high (75.83%), with 13.19%representing viral co-infections. Rotavirus detection rate (69.17%) was higher than that for other viral agents (15.83%). Both mono- and mixed infections were observed for RoV, AdV and NoVII, whereas NoVI was detected only in co-infection cases. Analysis of risk factors showed that acute gastroenteritis was detected more often in infants of age ˂1 year (73.53%) than in those 1 ≤ 2 years (22.55%) or > 2 years (3.92%) in age. Gender and age were not associated with the cases of co-infections (p˂0.05). The seasonality data indicated one peak of the infection occurring in January 2017 which has decreased consecutively in the subsequent two years. These results demonstrate the prevalence and co-occurrence of enteric viruses in cases of infantile diarrhoea in Nsukka. Further molecular characterization of enteric virus strains, especially noroviruses, in this region would contribute significantly to global epidemiological data.