Methods for Preparing Nucleosomes Containing Histone Variants
Methods for Preparing Nucleosomes Containing Histone Variants
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DOI：
10.1007/978-1-4939-8663-7_1
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发表时间：
2018-01-01
期刊：
影响因子：
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通讯作者：
Kurumizaka, Hitoshi
中科院分区：
文献类型：
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作者：
Kujirai, Tomoya;Arimura, Yasuhiro;Kurumizaka, Hitoshi
组蛋白变异是调控基因组DNA转录、修复、复制和重组的关键表观遗传学参与者。组蛋白变异掺入核小体诱导核小体的结构多样性，从而导致染色质结构的多功能性。这种由组蛋白变异产生的染色质多样性可能在基因的表观遗传调控中发挥核心作用。每种组蛋白变体都具有特定的生化和物理特性，因此制备方法复杂。本文介绍了以重组蛋白形式纯化人组蛋白变体的方法，并描述了含各种组蛋白变体的组蛋白复合物和核小体的制备方法。我们还详细描述了异型核小体的制备方法，它可能在某些生物现象中起作用。这些方法对生物化学、结构和生物物理学的研究都很有用。
Histone variants are key epigenetic players that regulate transcription, repair, replication, and recombination of genomic DNA. Histone variant incorporation into nucleosomes induces structural diversity of nucleosomes, consequently leading to the structural versatility of chromatin. Such chromatin diversity created by histone variants may play a central role in the epigenetic regulation of genes. Each histone variant possesses specific biochemical and physical characteristics, and thus the preparation methods are complicated. Here, we introduce the methods for the purification of human histone variants as recombinant proteins, and describe the preparation methods for histone complexes and nucleosomes containing various histone variants. We also describe the detailed method for the preparation of heterotypic nucleosomes, which may function in certain biological phenomena. These methods are useful for biochemical, structural, and biophysical studies.