Implementation of a Project-Based Molecular Biology Laboratory Emphasizing Protein Structure-Function Relationships in a Large Introductory Biology Laboratory Course
Implementation of a Project-Based Molecular Biology Laboratory Emphasizing Protein Structure-Function Relationships in a Large Introductory Biology Laboratory Course
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DOI：
10.1187/cbe.10-07-0085
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发表时间：
2011-03-01
影响因子：
3.7
通讯作者：
Kosinski-Collins, Melissa S.
中科院分区：
文献类型：
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作者：
Treacy, Daniel J.;Sankaran, Saumya M.;Kosinski-Collins, Melissa S.
在实验入门课程中，许多大学正在从具有可预测结果的传统实验室转向探究性的、基于项目的实验室课程。在这些实验室里，学生们被允许设计至少一部分自己的实验，并解释新的、未发现的数据。我们重新设计了布兰迪斯大学的入门生物学实验课程，将其改造为一个学期的基于项目的实验室，强调概念并包含科学探究的元素。在这个实验室中，学生们对编码人类γ D晶体蛋白（一种与白内障有关的人类晶状体蛋白）的基因进行了定点诱变实验，并评估了他们新创造的蛋白质相对于野生型晶体蛋白的稳定性。本实验室利用分子生物学的基本技术来强调DNA和蛋白质之间联系的重要性。这个项目实验室帮助学生参与自己的学习，提高了学生的批判性思维和分析能力，并促进了对生物学基础研究的兴趣。
In introductory laboratory courses, many universities are turning from traditional laboratories with predictable outcomes to inquiry-inspired, project-based laboratory curricula. In these labs, students are allowed to design at least some portion of their own experiment and interpret new, undiscovered data. We have redesigned the introductory biology laboratory course at Brandeis University into a semester-long project-based laboratory that emphasizes concepts and contains an element of scientific inquiry. In this laboratory, students perform a site-directed mutagenesis experiment on the gene encoding human gamma D crystallin, a human eye lens protein implicated in cataracts, and assess the stability of their newly created protein with respect to wild-type crystallin. This laboratory utilizes basic techniques in molecular biology to emphasize the importance of connections between DNA and protein. This project lab has helped engage students in their own learning, has improved students' skills in critical thinking and analysis, and has promoted interest in basic research in biology.