The murine perilipin gene: the lipid droplet-associated perilipins derive from tissue-specific, mRNA splice variants and define a gene family of ancient origin
The murine perilipin gene: the lipid droplet-associated perilipins derive from tissue-specific, mRNA splice variants and define a gene family of ancient origin
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DOI：
10.1007/s00335-01-2055-5
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发表时间：
2001-09-01
期刊：
影响因子：
2.5
通讯作者：
Kimmel, AR
中科院分区：
文献类型：
--
作者：
Lu, XY;Gruia-Gray, J;Kimmel, AR
Perilipins是一类细胞内中性脂滴储存蛋白，对急性蛋白激酶A介导的激素刺激有反应。Perilipin(Peri)的表达似乎仅限于脂肪细胞和类固醇生成细胞，其中细胞内的中性脂水解受蛋白激酶A的调节。我们分离了小鼠Peri基因座的cDNA集和重叠的基因组片段，并定位了染色体位置、转录起始点、多聚腺苷化位点和内含子/外显子连接。数据证实，Perilipins是由一个单拷贝基因编码的，具有选择性和组织特异性，mRNA剪接和聚腺苷酸化产生四种不同的蛋白质种类。Perilipin蛋白与22 kDa的氨基末端相同，具有不同长度的不同羧基末端序列。这些小鼠的基因组和转录图谱。Perilipin对于评估基因座内假定的内源性和靶向突变也是必不可少的。Perilipins的N-末端同一性区域定义了一个序列基序，我们称之为PAT，它与adrp和TIP47蛋白共享；此外，PAT结构域可能代表了脊椎动物和无脊椎动物类脂储存液滴(LSD)蛋白的一种新的、保守的模式。比较基因组学表明，相关的LSD基因在不同物种中的存在，如果蝇和网柄网柄菌。
The Perilipins are a family of intracellular neutral lipid droplet storage proteins that are responsive to acute protein kinase A-mediated, hormonal stimulation. Perilipin (Peri) expression appears to be limited to adipocytes and steroidogenic cells, in which intracellular neutral lipid hydrolysis is regulated by protein kinase A. We have isolated cDNA sets and overlapping genomic fragments of the murine Peri locus and mapped chromosomal location, transcription start sites, polyadenylylation sites, and intron/exon junctions. Data confirm that the Perilipins are encoded by a single-copy gene, with alternative and tissue-specific, mRNA splicing and polyadenylylation yielding four different protein species. The Perilipin proteins have identical similar to 22-kDa amino termini with distinct carboxyl terminal sequences of varying lengths. These genomic and transcriptional maps of murine. Perilipin are also essential for evaluating presumptive endogenous and targeted mutations within the locus. The N-terminal identity region of the Perilipins defines a sequence motif, which we term PAT, that is shared with the ADRP and TIP47 proteins; additionally, the PAT domain may represent a novel, conserved pattern for lipid storage droplet (LSD) proteins of vertebrates and invertebrates alike. Comparative genomics suggest the presence of related LSD genes in species as diverse as Drosophila and Dictyostelium.