Schistosoma japonicum protein SjP40 inhibits TGF-β1-induced activation of hepatic stellate cells
Schistosoma japonicum protein SjP40 inhibits TGF-β1-induced activation of hepatic stellate cells
复制标题
日本血吸虫蛋白SjP40抑制TGF-β1诱导的肝星状细胞活化
DOI：
10.1007/s00436-015-4663-0
复制
发表时间：
2015-11-01
影响因子：
2
通讯作者：
Duan, Yinong
中科院分区：
文献类型：
--
作者：
Sun, Xiaolei;Zhang, Lingbo;Duan, Yinong
SjP 40是日本血吸虫虫卵的主要抗原。在本研究中，作者研究了SjP 40在体外对转化生长因子-β 1（TGF-β 1）刺激的肝星状细胞（HSC）的影响。LX-2是一种永生化的人HSC系，在存在或不存在TGF-β 1的情况下用纯化的重组SjP 40（rSjP 40）处理。采用实时定量聚合酶链反应和蛋白质印迹法分析纤维化基因和TGF-β信号通路的信使核糖核酸和蛋白质。结果显示，rSjP 40显著降低了纤维化基因的表达。此外，rSjP 40还抑制TGF-β 1诱导的Smads和ERK蛋白的上调。我们还发现rSjP 40对HSC的作用类似于I型TGF-β受体抑制剂SB 431542。总之，数据表明，SjP 40减弱HSC活化，这可能是，至少部分地，通过抑制TGF-β和ERK信号通路介导的。
SjP40 is a major egg antigen of Schistosoma japonicum. In the present study, the authors investigated the effect of SjP40 in vitro on transforming growth factor-beta 1 (TGF-beta 1)- stimulated hepatic stellate cells (HSCs). LX-2, an immortalized human HSC line, was treated with purified recombinant SjP40 (rSjP40) in the presence or absence of TGF-beta 1. Quantitative real-time polymerase chain reaction and western blot analysis were performed to determine messenger ribonucleic acid and protein of fibrogenic genes and TGF-beta signaling pathway. The results showed that expression of fibrogenic genes was significantly reduced by rSjP40. Furthermore, rSjP40 also suppressed the TGF-beta 1-induced upregulation of Smads and ERK proteins. We also found that the effect of rSjP40 on HSCs was similar to SB431542, an inhibitor of type I TGF-beta receptor. In conclusion, the data suggest that SjP40 attenuates HSC activation, which might be, at least in part, mediated by inhibiting the TGF-beta and ERK signaling pathways.