Reliability of biomarkers of iron status, blood lipids, oxidative stress, vitamin D, C-reactive protein and fructosamine in two Dutch cohorts
Reliability of biomarkers of iron status, blood lipids, oxidative stress, vitamin D, C-reactive protein and fructosamine in two Dutch cohorts
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DOI：
10.1080/13547500600799748
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发表时间：
2006-07-01
期刊：
影响因子：
2.6
通讯作者：
Beuno-De-Mesquita, H. B.
中科院分区：
文献类型：
--
作者：
Al-Delaimy, W. K.;Jansen, E. H. J. M.;Beuno-De-Mesquita, H. B.
生物标志物在流行病学中被广泛使用，但很少有可靠性研究来评估使用这些生物标志物评估疾病-疾病关系的适当性。这项研究的目的是评估从两个荷兰中心（乌得勒支和比尔托芬）收集的血清中20种生物标志物的可靠性，这些中心参加了欧洲癌症和营养前瞻性调查（EPIC），时间间隔为几年。从Bilthoven的30名男性和乌得勒支的35名女性中采集了血液样本。铁蛋白、总铁、总铁结合能力、转铁蛋白饱和度、转铁蛋白、C-反应蛋白、胆红素、胆固醇、甘油三酯、载脂蛋白-A、载脂蛋白-B、高密度脂蛋白、低密度脂蛋白、尿酸、肌酐、活性氧代谢物、血浆的铁还原能力、蛋白硫醇氧化、果糖胺、在两个时间点从血液样品分析血清中的维生素D生物标志物。除C反应蛋白外，所有生物标志物的平均水平均未随时间发生实质性变化。尿酸、铁蛋白、肌酐、HDL和载脂蛋白-B水平始终显示男性和女性的最高可靠性（组内相关性=0.69-0.86）。在女性中，血浆铁还原能力和蛋白巯基氧化的可靠性较差;在男性中，铁相关生物标志物（血清铁蛋白除外）的可靠性较差。除了少数性别特异性差异外，20种生物标志物中的大多数表现良好，可以认为具有足够的可靠性，可用于未来的队列研究。
Biomarkers are widely used in epidemiology, yet there are few reliability studies to assess the appropriateness of using these biomarkers for the assessment of exposure-disease relationships. The aim of the study was to assess the reliability of 20 biomarkers in serum collected from two Dutch centres (Utrecht and Bilthoven) participating in the European Prospective Investigation into Cancer and Nutrition (EPIC) at two points several years apart. Blood samples were collected from 30 men from Bilthoven and 35 women from Utrecht. Ferritin, total iron, total iron-binding capacity, transferrin saturation, transferrin, C-reactive protein, bilirubin, cholesterol, triglycerides, apo lipoprotein-A, apo lipoprotein-B, high-density lipoproteins, low-density lipoproteins, uric acid, creatinine, reactive oxygen metabolites, the ferric-reducing ability of plasma, protein thiol oxidation, fructosamine, and vitamin D biomarkers in serum were analysed from the blood samples at the two points of time. For all biomarkers, except C-reactive protein, there were no substantial changes in the mean levels over time. Uric acid, ferritin, creatinine, HDL, and apo lipoprotein-B levels consistently showed the highest reliability for men and women (intra-class correlation =0.69-0.86). Among women, the ferric-reducing ability of plasma, and protein thiol oxidation had poor reliability; and among men iron-related biomarkers (except serum ferritin) had poor reliability. With the exception of a few gender-specific differences, most of the 20 biomarkers performed well and can be considered to have sufficient reliability to be used in future cohort studies.