Characterizing the impact of CD8 antibodies on class I MHC multimer binding
Characterizing the impact of CD8 antibodies on class I MHC multimer binding
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DOI：
10.4049/jimmunol.174.7.3986
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发表时间：
2005-04-01
影响因子：
4.4
通讯作者：
Jameson, SC
中科院分区：
文献类型：
--
作者：
Holman, PO;Walsh, ER;Jameson, SC
许多研究表明，CD8抗体影响I类MHC四聚体/多聚体与CD8(+) T细胞的结合，这导致了CD8直接参与多聚体结合的解释。相比之下，最近的一篇文章认为CD8抗体反而引起TCR分布的重组，因此对单独与TCR的多聚体结合有间接影响。我们通过测试CD8的作用和CD8抗体对正常和突变多聚体结合的影响来解决这些问题；抗原特异性小鼠T细胞我们的数据表明，在这个系统中，CD8抗体直接作用于CD8，只有当CD8能够与多聚体结合时才介导它们对多聚体结合的影响。这些数据强化了CD8在I类MHC多聚体结合中起积极和直接作用的范式。
Many studies have suggested that CD8 Abs affect the binding of class I MHC tetramers/multimers to CD8(+) T cells, which has led to the interpretation that CD8 participates directly in multimer binding. In contrast, a recent publication has argued that CD8 Abs instead cause reorganization of TCR distribution and hence have an indirect effect on multimer binding to the TCR alone. We address these issues by testing the role of CD8 and the impact of CD8 Abs on the binding of normal and mutant multimers; to Ag-specific mouse T cells. Our data suggest that, in this system, CD8 Abs act directly on CD8 and only mediate their effects on multimer binding when CD8 is capable of binding to the multimer. These data reinforce the paradigm that CD8 plays an active and direct role in binding of class I MHC multimers.