CD23 trimers are preassociated on the cell surface even in the absence of its ligand, IgE
CD23 trimers are preassociated on the cell surface even in the absence of its ligand, IgE
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DOI：
10.4049/jimmunol.172.2.1065
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发表时间：
2004-01-15
影响因子：
4.4
通讯作者：
Conrad, DH
中科院分区：
文献类型：
--
作者：
Kilmon, MA;Shelburne, AE;Conrad, DH
过敏性疾病由高水平的过敏原特异性IgE介导。免疫球蛋白E与免疫球蛋白E的低亲和力受体CD 23结合，产生负反馈信号，导致免疫球蛋白E产生减少。先前的研究已经表明，CD 23作为寡聚体缔合，并且高亲和力IgE与CD 23结合需要至少两个凝集素结构域的协同结合。我们以前已经表明，合作的结合是必需的调节IgE的生产。本研究描述了结合鼠CD 23的茎区的几种mAb的生产。其中19 G5在37 ℃时能抑制IgE/CD 23的相互作用，但在VC时不抑制。对这些抗体结合特性的分析表明，CD 23在高温（如37 ℃）下解离;然而，N末端是组成性结合的，表明部分解离，而不是完全解离。一个新的发现是，茎区，以前认为介导的三聚体协会，是不需要寡聚化。这些数据揭示了有关CD 23结构的重要信息，这些信息可能有助于调节IgE的产生。
Allergic disease is mediated by high levels of allergen-specific IgE. IgE binding to CD23, the low affinity receptor for IgE, results in a negative feedback signal leading to a decrease in IgE production. Previous studies have shown that CD23 associates as an oligomer and that cooperative binding of at least two lectin domains is required for high affinity IgE binding to CD23. We have previously shown that cooperative binding is required for regulation of IgE production. This study describes the production of several mAbs that bind the stalk region of murine CD23. One of the Abs, 19G5, inhibited the IgE/CD23 interaction at 37degreesC, but not at VC. Analysis of the binding properties of these Abs revealed that CD23 dissociates at high temperatures, such as 37degreesC; however, the N terminus is constitutively associated, indicating partial, rather than complete, dissociation. A novel finding was that the stalk region, previously thought to mediate trimer association, was not required for oligomerization. These data reveal important information about the structure of CD23 that may be useful in modulating IgE production.