Augmenter of liver regeneration attenuates acute rejection after rat liver transplantation
Augmenter of liver regeneration attenuates acute rejection after rat liver transplantation
复制标题
肝再生增强剂可减轻大鼠肝移植后的急性排斥反应
DOI：
10.1016/j.amjsurg.2015.10.036
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发表时间：
2016-07-01
影响因子：
3
通讯作者：
Gong, Jianping
中科院分区：
文献类型：
--
作者：
Chen, Yong;Liang, Shaoyong;Gong, Jianping
背景技术背景：方法：将雄性刘易斯和Brown-Norway（BN）大鼠分为同种异体移植组（Lewis to BN肝移植）、同系移植组（BN to BN）和ALR组（Lewis to BN，ALR，100 μ g/kg/d，术后肌肉注射）。在指定的时间处死大鼠以评估细胞因子产生、T细胞（TC）活化和凋亡。从移植物中分离枯否细胞（KCs）和TC以评估细胞因子表达。结果：（1）ALR处理可显著降低白细胞介素2（IL-2）和干扰素γ（IFN-γ）的表达，促进TC凋亡，延长移植物存活时间;（2）ALR组和同系移植组KCs中IL-10水平显著升高，TNF-α水平显著降低。与同种异体移植组相比，ALR α表达可抑制TC的增殖并诱导其凋亡;（3）ALR和KCs直接抑制TC的增殖和活化，并诱导TC凋亡。ALR在体内外均能抑制TC的增殖和功能，减轻肝移植术后的急性排斥反应。(C)2016 Elsevier Inc. All rights reserved.
BACKGROUND: The role of augmenter of liver regeneration (ALR) on liver transplantation immune regulation remains unknown.METHODS: Male Lewis and Brown-Norway (BN) rats were assigned to allograft group (Lewis-to-BN liver transplantation), isograft group (BN-to-BN), and ALR group (Lewis-to-BN, ALR, 100 mu g/kg/d, intramuscular injection postoperatively). Rats were sacrificed at indicated times for assessment of cytokines production, T-cell (TC) activation and apoptosis. Kupffer cells (KCs) and TCs were isolated from grafts to assess cytokine expression. Effect of ALR and KCs on TCs was monitored by co-culture of H-3-thymidine TCs.RESULTS: (1) Treatment with ALR significantly decreased interleukin-2 and interferon-gamma expression, promoted TC apoptosis, and prolonged the survival of allografts; (2) KCs in ALR group and isograft group that had significantly increased interleukin-10 and decreased tumor necrosis factor-alpha expression were able to inhibit TC proliferation and induce their apoptosis relative to KCs in the allograft group; (3) ALR and KCs directly inhibited TC proliferation and activation and induced TC apoptosis.CONCLUSIONS: ALR could inhibit TC proliferation and function both in vivo and in vitro and attenuate acute rejection after liver transplantation. (C) 2016 Elsevier Inc. All rights reserved.