Treatment of experimental viral myocarditis with interleukin-10
Treatment of experimental viral myocarditis with interleukin-10
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DOI：
10.1161/01.cir.100.10.1102
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发表时间：
1999-09-07
期刊：
影响因子：
37.8
通讯作者：
Sasayama, S
中科院分区：
文献类型：
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作者：
Nishio, R;Matsumori, A;Sasayama, S
背景-辅助性T细胞2型相关细胞因子白介素10具有多种免疫调节特性。然而，细胞因子在病毒性心肌炎中的作用尚不清楚。方法和结果-在脑心肌炎病毒(EMCV)所致的小鼠急性病毒性心肌炎模型中，我们研究了重组人IL-10(rhIL-10)的完全活性对小鼠细胞的影响。4周龄DBA/2小鼠接种EMCV(第0天)。从第0天开始给予重组人白介素10(10mgg/只)，每日1次，对照组仅给予赋形剂。于第14天测定存活率。第6天检测心肌组织病理学、细胞因子水平、心肌病毒浓度，竞争性聚合酶链式反应检测心肌诱导型一氧化氮合酶(INOS)基因表达水平。Rh IL-10组小鼠14天存活率(80%)显著高于对照组(30%，n=10)，P
Background-The T helper cell type 2-associated cytokine interleukin (IL)-10 has a variety of immunomodulatory properties. However, the effects of the cytokine on viral myocarditis remain unclear.Methods and Results-We studied the effects of recombinant human IL-10 (rhIL-10) fully active on mouse cells in a murine experimental model of acute viral myocarditis caused by the encephalomyocarditis virus (EMCV). Four-week-old DBA/2 mice were inoculated with EMCV (day 0). rhIL-10 (10 mu g/mouse) was administered once daily, starting on day 0, and control mice received vehicle only. Survival rates were determined on day 14. Myocardial histopathology, cytokine levels in the heart by ELISA assay, and myocardial virus concentration were examined on day 6, and the expression levels of myocardial inducible nitric oxide synthase (iNOS) mRNA were measured by competitive polymerase chain reaction. The 14-day survival in mice treated with rhIL-10 was significantly higher (80%) than in the control group (30%, n=10 in each, P