Heat Shock Protein 70 Expression Increased in Rabbit Müller Cells in the Ischemia-Reperfusion Model
Heat Shock Protein 70 Expression Increased in Rabbit Müller Cells in the Ischemia-Reperfusion Model
复制标题
缺血再灌注模型中兔 Müller 细胞中热休克蛋白 70 表达增加
作者：
T. Gohdo;H. Ueda;S. Ohno;H. Iijima;S. Tsukahara
在缺血再灌注模型中检测热休克蛋白70（HSP70）在兔视网膜中的表达。通过增加前房中的压力诱导缺血，并在再灌注后1或12 h收集视网膜。Western blot、激光共聚焦显微镜和免疫电镜检测HSP70的表达。Western blot结果显示，再灌注12 h后视网膜中HSP 70表达明显增加，与正常对照组和再灌注1 h相比差异有统计学意义（P <0.05）。共聚焦显微镜观察表明，HSP70在Müller细胞中是组成性的，并且其表达在再灌注12 h后增加。电镜显示再灌注后1h糖原样颗粒明显减少，内质网明显增多。然而，再灌注12小时后，糖原样颗粒的密度恢复，免疫电镜显示HSP70与Müller细胞的细胞器相关。综上所述，这些数据表明，Müller细胞中的HSP 70在适应应激中起作用。
Heat shock protein 70 (HSP70) expression in the rabbit retina was evaluated in the ischemia-reperfusion model. Ischemia was induced by increasing the pressure in the anterior chamber, and the retinas were collected 1 or 12 h after reperfusion. HSP70 expression was examined by Western blot, confocal laser scanning microscopy and immunoelectron microscopy. Western blot showed that HSP70 was significantly increased in the retina 12 h after reperfusion, compared with normal control or 1 h after reperfusion. Confocal microscopy demonstrated that HSP70 was constitutive in Müller cells and that its expression was increased 12 h after reperfusion. Electron microscopy showed that glycogen-like granules were significantly decreased with prominent endoplasmic reticulum 1 h after reperfusion. Twelve hours after reperfusion, however, the density of glycogen-like granules was restored, and immunoelectron microscopy indicated that HSP70 was associated with the organelles of the Müller cells. Taken together, these data suggest that HSP70 in Müller cells plays a role in accommodation to stress.
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影响因子：
2.9
作者：
Robert J. Schumacher;W J Hansen;Brian C. Freeman;Emad S. Alnemri;Gerald Litwack;D. Toft
通讯作者：
Robert J. Schumacher;W J Hansen;Brian C. Freeman;Emad S. Alnemri;Gerald Litwack;D. Toft
DOI：
10.1615/critreveukargeneexpr.v4.i1.10
发表时间：
1994
影响因子：
1.6
作者：
E. Little;Meera Ramakrishnan;Binayak Roy;G. Gazit;Amy S. Lee
通讯作者：
E. Little;Meera Ramakrishnan;Binayak Roy;G. Gazit;Amy S. Lee
高温和缺氧会增加视网膜神经节细胞对缺氧和兴奋性毒性的耐受性。
影响因子：
4.4
作者：
Caprioli,J;Kitano,S;Morgan,JE
通讯作者：
Morgan,JE
影响因子：
--
作者：
S. A. Gordon;R. Hoffman;R. Simmons;H. Ford
通讯作者：
S. A. Gordon;R. Hoffman;R. Simmons;H. Ford