Identifying QTL for high-temperature adult-plant resistance to stripe rust (Puccinia striiformis f. sp tritici) in the spring wheat (Triticum aestivum L.) cultivar 'Louise'
Identifying QTL for high-temperature adult-plant resistance to stripe rust (Puccinia striiformis f. sp tritici) in the spring wheat (Triticum aestivum L.) cultivar 'Louise'
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DOI：
10.1007/s00122-009-1114-2
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发表时间：
2009-10-01
影响因子：
5.4
通讯作者：
Kidwell, K. K.
中科院分区：
文献类型：
--
作者：
Carter, Arron Hyrum;Chen, X. M.;Kidwell, K. K.
随着时间的推移，小麦（Triticum aestivum L.）中许多针对条锈病（Puccinia striiformis f. sp. tritici）的单一全阶段抗性基因因病原体的种族变化而被规避。相比之下，高温成株抗性（HTAP）仅在成株阶段和气温温暖时表达，可提供针对条锈病的持久保护。我们的目标是确定春小麦品种“Louise”中 HTAP 抗条锈病的主要数量性状基因座 (QTL)。作图群体由 188 个来自 Louise（抗性）和“Penawawa”（易感）杂交的重组自交系 (RIL) 组成。 2007 年和 2008 年，在美国太平洋西北地区五个地点的重复田间试验中，评估了 F-5:6 品系在自然感染下的条锈病反应。每个地点记录每个 RIL 的感染类型 (IT) 和疾病严重程度 2-4 次。在所有环境中，易感亲代 Penawawa 在所有评估的生长阶段的 IT 等级为 6 至 8。相比之下，抗性亲本 Louise 在整个生长阶段的 IT 等级为 2 或 3。 IT值的分布是双峰的，表明单个主基因正在影响该性状。使用 295 个多态性简单序列重复和 1 个单核苷酸多态性标记对亲本和 RIL 群体进行评估。在 2BS 染色体上检测到一个主要 QTL，命名为 QYrlo.wpg-2BS，与 Louise 的 HTAP 抗性相关（LOD 评分范围为 5.5 至 62.3，不同地点和年份），位于 Xwmc474 和 Xgwm148 两侧的 16.9 cM 区域内。与 QYrlo.wpg-2BS 相关的 SSR 标记目前用于基于标记的正向育种策略，将目标区域转移到适应的种质中，以提高所得品种的抗性持久性。
Over time, many single, all-stage resistance genes to stripe rust (Puccinia striiformis f. sp. tritici) in wheat (Triticum aestivum L.) are circumvented by race changes in the pathogen. In contrast, high-temperature, adult-plant resistance (HTAP), which only is expressed during the adult-plant stage and when air temperatures are warm, provides durable protection against stripe rust. Our objective was to identify major quantitative trait loci (QTL) for HTAP resistance to stripe rust in the spring wheat cultivar 'Louise'. The mapping population consisted of 188 recombinant inbred lines (RIL) from a Louise (resistant) by 'Penawawa' (susceptible) cross. F-5:6 lines were evaluated for stripe rust reaction under natural infection in replicated field trials at five locations in the US Pacific Northwest in 2007 and 2008. Infection type (IT) and disease severity were recorded for each RIL 2-4 times per location. In all environments, Penawawa, the susceptible parent, was rated with an IT ranging from 6 to 8 at all growth stages evaluated. In contrast, Louise, the resistant parent, was rated with an IT of 2 or 3 across growth stages. Distribution of IT values was bimodal, indicating a single major gene was affecting the trait. The parents and RIL population were evaluated with 295 polymorphic simple sequence repeat and one single nucleotide polymorphism markers. One major QTL, designated QYrlo.wpg-2BS, associated with HTAP resistance in Louise, was detected on chromosome 2BS (LOD scores ranging from 5.5 to 62.3 across locations and years) within a 16.9 cM region flanked by Xwmc474 and Xgwm148. SSR markers associated with QYrlo.wpg-2BS are currently being used in marker-based forward breeding strategies to transfer the target region into adapted germplasm to improve the durability of resistance in resulting cultivars.