Communication: Near edge x-ray absorption fine structure spectroscopy of aqueous adenosine triphosphate at the carbon and nitrogen K-edges
Communication: Near edge x-ray absorption fine structure spectroscopy of aqueous adenosine triphosphate at the carbon and nitrogen K-edges
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DOI：
10.1063/1.3478548
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发表时间：
2010-09-14
影响因子：
4.4
通讯作者：
Saykally, Richard J.
中科院分区：
文献类型：
--
作者：
Kelly, Daniel N.;Schwartz, Craig P.;Saykally, Richard J.
采用近边x射线吸收精细结构（NEXAFS）技术研究了三磷酸腺苷在液体微射流中的水合作用。总电子产率谱记录为浓度、pH和钠、镁、铜离子（Na+/Mg2+/Cu2+）存在的函数。在腺嘌呤环质子化时观察到明显的光谱变化，但在促进pi堆积的条件下，如高浓度或Mg2+的存在，则没有明显的变化，这表明NEXAFS对这种现象不敏感。分子内与Cu2+的球内结合确实产生了可观察到的氮谱增宽，而与Mg2+的球外结合则没有。(c) 2010年美国物理研究所。(doi: 10.1063/1.3478548)
Near edge x-ray absorption fine structure (NEXAFS) spectroscopy at the nitrogen and carbon K-edges was used to study the hydration of adenosine triphosphate in liquid microjets. The total electron yield spectra were recorded as a function of concentration, pH, and the presence of sodium, magnesium, and copper ions (Na+/Mg2+/Cu2+). Significant spectral changes were observed upon protonation of the adenine ring, but not under conditions that promote pi-stacking, such as high concentration or presence of Mg2+, indicating that NEXAFS is insensitive to the phenomenon. Intramolecular inner-sphere association of Cu2+ did create observable broadening of the nitrogen spectrum, whereas outer-sphere association with Mg2+ did not. (c) 2010 American Institute of Physics. [doi:10.1063/1.3478548]