Transchip: Single-molecule detection of transcriptional elongation complexes
Transchip: Single-molecule detection of transcriptional elongation complexes
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DOI：
10.1016/j.ab.2006.10.042
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发表时间：
2007-02-01
影响因子：
2.9
通讯作者：
Schwartz, David C.
中科院分区：
文献类型：
--
作者：
Wu, Tian;Schwartz, David C.
一种新的单分子系统TransChip被开发出来，用于分析转录产物的基因组起源。噬菌体T7 RNA聚合酶及其启动子被用于模型系统中，并在其沿单模板DNA分子的位置成像和检测得到的RNA。TransChip系统借鉴了光学映射的关键方面，这是一个单分子系统，能够从单个DNA分子构建整个基因组的高分辨率有序限制图。通过对数百个单分子模板/转录本复合体的统计分析，TransChip能够分析启动子的位置和强度、转录反应的方向和过程以及转录的终止。这些新的结果表明，新的系统可以作为一个高通量的平台，在大规模基因组范围内研究转录事件。(C)2006 Elsevier Inc.保留所有权利。
A new single-molecule system, Transchip, was developed for analysis of transcription products at their genomic origins. The bacteriophage T7 RNA polymerase and its promoters were used in a model system, and resultant RNAs were imaged and detected at their positions along single template DNA molecules. The Transchip system has drawn from critical aspects of Optical Mapping, a single-molecule system that enables the construction of high-resolution ordered restriction maps of whole genomes from single DNA molecules. Through statistical analysis of hundreds of single-molecule template/transcript complexes, Transchip enables analysis of the locations and strength of promoters, the direction and processivity of transcription reactions, and the termination of transcription. These novel results suggest that the new system may serve as a high-throughput platform to investigate transcriptional events on a large genome-wide scale. (c) 2006 Elsevier Inc. All rights reserved.