Rapid detection of Lassa virus by reverse transcription-loop-mediated isothermal amplification.
Rapid detection of Lassa virus by reverse transcription-loop-mediated isothermal amplification.
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DOI：
10.1111/j.1348-0421.2010.00286.x
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发表时间：
2011-01
影响因子：
2.6
通讯作者：
Yasuda J
中科院分区：
文献类型：
--
作者：
Fukuma A;Kurosaki Y;Morikawa Y;Grolla A;Feldmann H;Yasuda J
本研究建立了一种简便的一步反转录-环介导等温扩增(RT-LAMP)方法快速检测拉萨病毒(LASV)。这两套引物旨在检测在塞拉利昂和尼日利亚东北部传播的LASV。RT-LAMP方法能在25分钟内检测到100个拷贝的体外转录的人工LASV RNA。该方法还使用从细胞培养培养的病毒中提取的完整的病毒RNA进行了评估，并证实对LASV具有高度的特异性。本研究建立的RT-LAMP方法对LASV的检测具有快速、简便、高度特异的特点，但其灵敏度略低于实时RT-PCR。此外，由于RT-LAMP检测不需要使用复杂的设备，因此它将有利于发展中国家的LASV感染的临床诊断。它还可用于生物恐怖主义袭击期间故意释放的情况，或用于疾病暴发的流行病学监测。
In this study, a simple one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of Lassa virus (LASV) was established. The two primer sets were designed to detect LASV circulating in Sierra Leone and northeastern Nigeria. The RT-LAMP assay using these primer sets was able to detect 100 copies of the in vitro transcribed artificial LASV RNA within 25 min. The assay was also evaluated using intact viral RNA extracted from cell culture-propagated viruses and confirmed to be highly specific for LASV. The RT-LAMP assay developed in this study is rapid, simple, and highly specific for the detection of LASV, although its sensitivity is slightly lower than that of real-time RT-PCR. In addition, because the RT-LAMP assay does not require the use of sophisticated equipment, it would be advantageous for clinical diagnosis of LASV infection in developing countries. It might also be employed in cases of deliberate release during bioterrorism attacks or in epidemiological surveillance for disease outbreaks.
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影响因子：
158.5
作者：
MCCORMICK, JB;KING, IJ;BELMONTWILLIAMS, R
通讯作者：
BELMONTWILLIAMS, R
影响因子：
9.4
作者：
Parida, M;Posadas, G;Morita, K
通讯作者：
Morita, K
影响因子：
9.4
作者：
Bausch, DG;Rollin, PE;Ksiazek, TG
通讯作者：
Ksiazek, TG
影响因子：
9.4
作者：
Drosten, C;Göttig, S;Günther, S
通讯作者：
Günther, S
影响因子：
5.4
作者：
Bowen, MD;Rollin, PE;Nichol, ST
通讯作者：
Nichol, ST