Characterization of new members of the pregnancy-specific beta 1-glycoprotein family.
Characterization of new members of the pregnancy-specific beta 1-glycoprotein family.
复制标题
妊娠特异性 β1-糖蛋白家族新成员的表征。
DOI：
10.1007/bf00230182
复制
发表时间：
1991
影响因子：
4.3
通讯作者：
Tease,LA
中科院分区：
文献类型：
--
作者：
Chan,WY;Zheng,QX;McMahon,J;Tease,LA
从人足月胎盘cDNA文库中分离到3个妊娠特异性β1-糖蛋白（PSG）家族成员。这三种cdna编码的蛋白质具有相似的结构域。有34个氨基酸的先导序列，其后是109个氨基酸的n结构域。紧接在n结构域之后的是一个或两个重复的包含93个氨基酸的a结构域，一个包含85个氨基酸的b结构域和一个可变大小的c结构域。这些蛋白质是高度亲水的。然而，其中一个具有81个氨基酸的c结构域，该结构域非常疏水，可能作为膜附着位点。假定的细胞-细胞识别三肽，Arg-Gly-Asp，存在于两种蛋白质的n结构域。在HeLa细胞中发现了其中一个cdna的部分序列，而在胎儿肝脏中发现了与其中两个cdna高度同源的cdna。基于其结构，提出了PSG蛋白的功能作用。
Three cDNAs encoding members of the pregnancy-specific β1-glycoprotein (PSG) family were isolated from human term placental cDNA library. All three cDNAs encode proteins with similar domain structure. There is a leader sequence of 34 amino acids followed by an N-domain of 109 amino acids. Immediately after the N-domain are one or two copies of a repeating A-domain of 93 amino acids, a B-domain of 85 amino acids and a C-domain of variable size. The proteins are highly hydrophilic. However, one of them has an 81-amino acid C-domain which is very hydrophobic and could potentially serve as a membrane attachment site. The putative cell-cell recognition tripeptide, Arg-Gly-Asp, is present in the N-domain of two of the proteins. Partial sequence of one of the cDNAs has been found in HeLa cells while cDNAs highly homologous to two of the cDNAs have been found in the fetal liver. Functional roles of the PSG proteins basing on their structure are proposed.