Phospholipase activity in Cryptococcus neoformans: A new virulence factor?
Phospholipase activity in Cryptococcus neoformans: A new virulence factor?
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DOI：
10.1093/infdis/175.2.414
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发表时间：
1997-02-01
影响因子：
6.4
通讯作者：
Sorrell, TC
中科院分区：
文献类型：
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作者：
Chen, SCA;Muller, M;Sorrell, TC
将50株新生隐球菌接种到蛋黄琼脂上，对其胞外磷脂酶的产生进行了检测，其中49株产生了磷脂酶活性的克隆周围沉淀物。对4株临床分离株培养上清液中的磷脂酶B(PLB)、溶血磷脂酶和溶血磷脂酶-转酰基酶活性进行放射分析。菌落直径与菌落加琼脂沉淀物的比值(PZ)与原球蛋白活性相关。12株环境分离株和13株临床分离株的磷脂酶产量相似。加蒂尔。新生隐球菌变种的环境菌株。新生杆菌(n=8)在72 h比17株临床分离株产生更多的磷脂酶(平均为0.57比0.72；P<；0.01)，而在96 h时PZ值相似。4株高、中、低磷脂酶活性的BALB/c小鼠静脉接种后，肺和脑中隐球菌的定量结果表明，磷脂酶活性与毒力之间存在相关性。新生假单胞菌分泌的磷脂酶可能与毒力有关。
Fifty isolates of Cryptococcus neoformans were examined for extracellular phospholipase production after inoculation onto egg yolk agar; 49 produced a pericolonial precipitate indicative of phospholipase activity. Phospholipase B (PLB), lysophospholipase, and lysophospholipase-transacylase activities were identified by radiometric analysis in supernatants from 4 clinical isolates. The ratio of colony diameter to colony plus precipitate on agar (Pz) correlated with PLB activity. Phospholipase production was similar in 12 environmental and 13 clinical isolates of C. neoformans var. gattii. Environmental strains of C. neoformans var. neoformans (n = 8) produced more phospholipase at 72 h than did 17 clinical isolates (mean Pt, 0.57 vs. 0.72; P < .01); however, Pz values were similar at 96 h. Quantitation of cryptococci in the lungs and brains of BALB/c mice inoculated intravenously with 4 strains expressing high, intermediate, or low phospholipase activity revealed a correlation between phospholipase activity and virulence. Phospholipases secreted by C. neoformans may be implicated in virulence.