KIF3A is essential for sperm tail formation and manchette function
KIF3A is essential for sperm tail formation and manchette function
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DOI：
10.1016/j.mce.2013.06.030
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发表时间：
2013-09-05
影响因子：
4.1
通讯作者：
Sironen, Anu
中科院分区：
文献类型：
--
作者：
Lehti, Mari S.;Kotaja, Noora;Sironen, Anu
KIF3A 运动蛋白负责鞭毛内运输，这是纤毛细胞轴丝形成过程中蛋白质递送所必需的。 KIF3A 在精子发生过程中的功能尚不清楚。在这项研究中，我们发现 KIF3A 的缺失会严重损害精子尾部的形成，有趣的是，它还会影响 manchette 组织和精子头部的形状。我们的结果证明了控制体细胞纤毛形成的机制与精子特异性鞭毛形成的机制之间的相似性。此外，这项研究揭示了 KIF3A 作为精子发生的重要调节因子，并强调了 KIF3A 在维持男性生育能力方面的关键作用。我们还发现了 KIF3A 的几个新的相互作用伙伴，包括减数分裂特异性核结构蛋白 1 (MNS1)，它与 KIF3A 共定位于精子尾部的 manchette 和主要部分。这项研究强调了 KIF3A 介导的微管运输在精子发育和男性生育能力中的重要作用。 (c) 2013 Elsevier Ireland Ltd. 保留所有权利。
KIF3A motor protein is responsible for intraflagellar transport, which is required for protein delivery during axoneme formation in ciliated cells. The function of KIF3A during spermatogenesis is not known. In this study, we show that depletion of KIF3A causes severe impairments in sperm tail formation and interestingly, it also affects manchette organization and the shaping of sperm heads. Our results demonstrate the analogy between the mechanisms governing the formation of cilia in somatic cells and the formation of spermatozoa-specific flagella. Furthermore, this study reveals KIF3A as an important regulator of spermatogenesis and emphasizes the crucial role of KIF3A in maintaining male fertility. We also identified several novel interacting partners for KIF3A, including meiosis-specific nuclear structural protein 1 (MNS1) that colocalizes with KIF3A in the manchette and principal piece of the sperm tail. This study highlights the essential role of KIF3A-mediated microtubular transport in the development of spermatozoa and male fertility. (c) 2013 Elsevier Ireland Ltd. All rights reserved.