Introgression of opaque2 into Waxy Maize Causes Extensive Biochemical and Proteomic Changes in Endosperm.
Introgression of opaque2 into Waxy Maize Causes Extensive Biochemical and Proteomic Changes in Endosperm.
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DOI：
10.1371/journal.pone.0158971
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Zhang S
中科院分区：
文献类型：
--
作者：
Zhou Z;Song L;Zhang X;Li X;Yan N;Xia R;Zhu H;Weng J;Hao Z;Zhang D;Yong H;Li M;Zhang S
湿玉米以其优良的性状和经济价值在国内外广泛种植。但其赖氨酸含量较低，不能满足人畜的营养需求。本研究利用分子标记辅助选择（MAS）技术，将opaque 2（o2）等位基因导入糯玉米品系赵OP-6/O2 O2，成功改良了糯玉米的赖氨酸含量和品质。转录本丰度分析表明，wx 1在Zhao OP-6/o2 o2和Zhao OP-6/o2 o2中的表达水平没有差异。然而，赵OP-6/o2 o2的特点是一个表型的硬和玻璃质的籽粒和积累的蛋白体在较小的尺寸（三分之一的父母），但在更多的。生化分析表明，Zhao OP-6/o2 o2比Zhao OP-6/o2 o2的游离氨基酸减少了16.7%，尤其是糖酵解中间产物，但赖氨酸含量增加了51.6%（0.47%比0.31%）。赵OP-6/o2 o2的支链淀粉含量为98.5%，显著高于赵OP-6/O2 O2的97.7%。蛋白质组学分析表明，o2基因渗入不仅降低了除27-kDa γ-玉米醇溶蛋白外的其它玉米醇溶蛋白的积累，而且影响了与氨基酸合成、淀粉-蛋白平衡、胁迫反应和信号转导有关的其它蛋白质。本研究为深入了解opaque 2基因导入糯玉米胚乳中的代谢变化提供了一个有趣的视角。
Waxy maize is prevalently grown in China and other countries due to the excellent characters and economic value. However, its low content of lysine can’t meet the nutritional requirements of humans and livestock. In the present study, we introgressed the opaque2 (o2) allele into waxy maize line Zhao OP-6/O2O2 by using marker-assisted selection (MAS) technique and successfully improved the lysine content and quality of waxy maize. Transcript abundance analysis indicated that the wx1 expression levels had no difference between Zhao OP-6/o2o2 and Zhao OP-6/O2O2. However, Zhao OP-6/o2o2 was characterized by a phenotype of hard and vitreous kernels and accumulation of protein bodies at smaller size (one third of that of parents) but in larger numbers. Biochemical analyses showed that Zhao OP-6/o2o2 had 16.7% less free amino acids than Zhao OP-6/O2O2, especially those derived from glycolytic intermediates, but its content of lysine was increased by 51.6% (0.47% vs. 0.31%). The content of amylopectin was 98.5% in Zhao OP-6/o2o2, significantly higher than that in Zhao OP-6/O2O2 (97.7%). Proteomic analyses indicated that o2 introgression not only decreased the accumulation of various zein proteins except for 27-kDa γ-zein, but also affected other endosperm proteins related to amino acid biosynthesis, starch-protein balance, stress response and signal transduction. This study gives us an intriguing insight into the metabolism changes in endosperm of waxy maize introgressed with opaque2.